FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1509631808> ?p ?o ?g. }

• W1509631808 abstract "An integral membrane protease was solubilized and purified to homogeneity from rat submaxillary mitochondria. The purified enzyme could coagulate rabbit plasma. The molecular mass of the enzyme is 22 kDa on SDS-polyacrylamide gel electrophoresis under reducing conditions and 24 kDa on gel filtration on a Sephadex G-100 column. Its isoelectric point is 4.2-4.25. Enzyme activity is strongly inhibited by diisopropyl fluorophosphate, soybean trypsin inhibitor, benzamidine, aprotinin, and antipain, suggesting the enzyme as a serine protease. Its pH optimum for activity is 8.5. Zn2+ is strongly inhibitory; at 1 mM concentration it produced 72% inhibition. The enzyme is active toward different synthetic substrates (p-nitroanilide derivatives) containing Arg at the P1 position with blocked NH2 terminus. Kcat/Km was highest with the substrate N-Bz-Pro-Arg-pNa (where Bz is benzoyl and pNA is paranitroanilide). The purified enzyme coagulates rabbit plasma in a dose-dependent manner. Plasma coagulation by the enzyme is completely blocked in the presence of aprotinin or soybean trypsin inhibitor, suggesting that protease activity is required for this coagulation reaction. Antibody raised against the purified enzyme inhibits the plasma coagulation initiated by the enzyme. The enzyme can correct the prolonged clotting time of factor X-deficient human plasma but is unable to convert purified fibrinogen to fibrin clots, indicating factor Xa-like activity of the enzyme. The enzyme has the ability to activate prothrombin. Several properties of the enzyme distinguish it from other reported submaxillary proteases." @default.
• W1509631808 created "2016-06-24" @default.
• W1509631808 creator A5008348723 @default.
• W1509631808 creator A5009534181 @default.
• W1509631808 creator A5036993148 @default.
• W1509631808 creator A5044614687 @default.
• W1509631808 date "1994-06-01" @default.
• W1509631808 modified "2023-09-29" @default.
• W1509631808 title "A new blood-coagulating protease in mitochondrial membranes of rat submaxillary glands. Purification and characterization of protease and its blood-coagulating activity." @default.
• W1509631808 cites W141217221 @default.
• W1509631808 cites W1531765158 @default.
• W1509631808 cites W1559967710 @default.
• W1509631808 cites W1579116711 @default.
• W1509631808 cites W1587682317 @default.
• W1509631808 cites W1749592677 @default.
• W1509631808 cites W1845361985 @default.
• W1509631808 cites W1907990126 @default.
• W1509631808 cites W1913710500 @default.
• W1509631808 cites W1966497279 @default.
• W1509631808 cites W1975195021 @default.
• W1509631808 cites W1980235078 @default.
• W1509631808 cites W1997956070 @default.
• W1509631808 cites W2010200503 @default.
• W1509631808 cites W2016353825 @default.
• W1509631808 cites W2020452653 @default.
• W1509631808 cites W2021968709 @default.
• W1509631808 cites W2023196534 @default.
• W1509631808 cites W2039571655 @default.
• W1509631808 cites W2047953018 @default.
• W1509631808 cites W2048517328 @default.
• W1509631808 cites W2060322869 @default.
• W1509631808 cites W2062776307 @default.
• W1509631808 cites W2077841302 @default.
• W1509631808 cites W2088805142 @default.
• W1509631808 cites W2089030687 @default.
• W1509631808 cites W2092165212 @default.
• W1509631808 cites W2094967976 @default.
• W1509631808 cites W2100837269 @default.
• W1509631808 cites W2115656257 @default.
• W1509631808 cites W2171953643 @default.
• W1509631808 cites W2192299483 @default.
• W1509631808 cites W2274787163 @default.
• W1509631808 cites W4293247451 @default.
• W1509631808 doi "https://doi.org/10.1016/s0021-9258(17)33997-2" @default.
• W1509631808 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8206926" @default.
• W1509631808 hasPublicationYear "1994" @default.
• W1509631808 type Work @default.
• W1509631808 sameAs 1509631808 @default.
• W1509631808 citedByCount "5" @default.
• W1509631808 crossrefType "journal-article" @default.
• W1509631808 hasAuthorship W1509631808A5008348723 @default.
• W1509631808 hasAuthorship W1509631808A5009534181 @default.
• W1509631808 hasAuthorship W1509631808A5036993148 @default.
• W1509631808 hasAuthorship W1509631808A5044614687 @default.
• W1509631808 hasBestOaLocation W15096318081 @default.
• W1509631808 hasConcept C141071460 @default.
• W1509631808 hasConcept C141814087 @default.
• W1509631808 hasConcept C181199279 @default.
• W1509631808 hasConcept C185592680 @default.
• W1509631808 hasConcept C2775883766 @default.
• W1509631808 hasConcept C2776714187 @default.
• W1509631808 hasConcept C2776873056 @default.
• W1509631808 hasConcept C2777807008 @default.
• W1509631808 hasConcept C2779288520 @default.
• W1509631808 hasConcept C2779832904 @default.
• W1509631808 hasConcept C2780340462 @default.
• W1509631808 hasConcept C2780799893 @default.
• W1509631808 hasConcept C2781378782 @default.
• W1509631808 hasConcept C28212737 @default.
• W1509631808 hasConcept C41183919 @default.
• W1509631808 hasConcept C43617362 @default.
• W1509631808 hasConcept C50476208 @default.
• W1509631808 hasConcept C55493867 @default.
• W1509631808 hasConcept C56856141 @default.
• W1509631808 hasConcept C71924100 @default.
• W1509631808 hasConcept C96294017 @default.
• W1509631808 hasConceptScore W1509631808C141071460 @default.
• W1509631808 hasConceptScore W1509631808C141814087 @default.
• W1509631808 hasConceptScore W1509631808C181199279 @default.
• W1509631808 hasConceptScore W1509631808C185592680 @default.
• W1509631808 hasConceptScore W1509631808C2775883766 @default.
• W1509631808 hasConceptScore W1509631808C2776714187 @default.
• W1509631808 hasConceptScore W1509631808C2776873056 @default.
• W1509631808 hasConceptScore W1509631808C2777807008 @default.
• W1509631808 hasConceptScore W1509631808C2779288520 @default.
• W1509631808 hasConceptScore W1509631808C2779832904 @default.
• W1509631808 hasConceptScore W1509631808C2780340462 @default.
• W1509631808 hasConceptScore W1509631808C2780799893 @default.
• W1509631808 hasConceptScore W1509631808C2781378782 @default.
• W1509631808 hasConceptScore W1509631808C28212737 @default.
• W1509631808 hasConceptScore W1509631808C41183919 @default.
• W1509631808 hasConceptScore W1509631808C43617362 @default.
• W1509631808 hasConceptScore W1509631808C50476208 @default.
• W1509631808 hasConceptScore W1509631808C55493867 @default.
• W1509631808 hasConceptScore W1509631808C56856141 @default.
• W1509631808 hasConceptScore W1509631808C71924100 @default.
• W1509631808 hasConceptScore W1509631808C96294017 @default.
• W1509631808 hasLocation W15096318081 @default.
• W1509631808 hasOpenAccess W1509631808 @default.
• W1509631808 hasPrimaryLocation W15096318081 @default.

• next