Matches in SemOpenAlex for { <https://semopenalex.org/work/W1509651750> ?p ?o ?g. }
- W1509651750 endingPage "224" @default.
- W1509651750 startingPage "218" @default.
- W1509651750 abstract "It is important to establish the solvent and its method of use that will optimise the extraction of testosterone from faeces of the particular animal species under study. This will require a minimum of six faecal samples that have each received the same amount of radioactive testosterone, or tracer (approximately 10 000 dpm), which are then exposed to the different solvents. After the extraction procedure, an exact fraction of the solvent is measured for radioactivity, in order to calculate the recovery of the tracer added to the medium. The most common solvents used are ethanol or diethyl ether. The extraction method for diethyl ether is to add ten volumes of solvent, mix by vortexing for 1 min, and count an aliquot (calculated exactly) of the supernatant for radioactivity. The other extraction method, using ethanol, usually requires ten volumes of 90% ethanol added to the faeces, followed by boiling for 20 min, but the concentration of water in the ethanol may need to be varied between 80 and 90% in order to improve the extraction of steroid. Again, this depends on the species under study. The protocol below outlines an ethanolic extraction procedure, which recovered 90% of testosterone from our study animal." @default.
- W1509651750 created "2016-06-24" @default.
- W1509651750 creator A5049625457 @default.
- W1509651750 date "2003-04-24" @default.
- W1509651750 modified "2023-10-14" @default.
- W1509651750 title "Radioimmunoassay of testosterone in faeces" @default.
- W1509651750 cites W107012698 @default.
- W1509651750 cites W133960292 @default.
- W1509651750 cites W138270912 @default.
- W1509651750 cites W143640392 @default.
- W1509651750 cites W14495884 @default.
- W1509651750 cites W1500257575 @default.
- W1509651750 cites W1530144046 @default.
- W1509651750 cites W1532872724 @default.
- W1509651750 cites W1533500309 @default.
- W1509651750 cites W1539095425 @default.
- W1509651750 cites W154909065 @default.
- W1509651750 cites W1555875984 @default.
- W1509651750 cites W1559674022 @default.
- W1509651750 cites W1599282489 @default.
- W1509651750 cites W16175639 @default.
- W1509651750 cites W1681017205 @default.
- W1509651750 cites W1773226983 @default.
- W1509651750 cites W1808407909 @default.
- W1509651750 cites W1818683167 @default.
- W1509651750 cites W1836992725 @default.
- W1509651750 cites W1845521845 @default.
- W1509651750 cites W1868564979 @default.
- W1509651750 cites W1883239855 @default.
- W1509651750 cites W1887080363 @default.
- W1509651750 cites W1909636415 @default.
- W1509651750 cites W1940912130 @default.
- W1509651750 cites W1963611815 @default.
- W1509651750 cites W1963714593 @default.
- W1509651750 cites W1964104682 @default.
- W1509651750 cites W1964140841 @default.
- W1509651750 cites W1964161720 @default.
- W1509651750 cites W1964415139 @default.
- W1509651750 cites W1964698667 @default.
- W1509651750 cites W1964775268 @default.
- W1509651750 cites W1965044937 @default.
- W1509651750 cites W1965079314 @default.
- W1509651750 cites W1965370892 @default.
- W1509651750 cites W1965486589 @default.
- W1509651750 cites W1965715416 @default.
- W1509651750 cites W1965931694 @default.
- W1509651750 cites W1966822536 @default.
- W1509651750 cites W1967611059 @default.
- W1509651750 cites W1968215587 @default.
- W1509651750 cites W1969216851 @default.
- W1509651750 cites W1969694110 @default.
- W1509651750 cites W1970403163 @default.
- W1509651750 cites W1971384034 @default.
- W1509651750 cites W1972187441 @default.
- W1509651750 cites W1972391606 @default.
- W1509651750 cites W1972853535 @default.
- W1509651750 cites W1973941232 @default.
- W1509651750 cites W1974134775 @default.
- W1509651750 cites W1975291023 @default.
- W1509651750 cites W1976113460 @default.
- W1509651750 cites W1976304819 @default.
- W1509651750 cites W1976397277 @default.
- W1509651750 cites W1976979766 @default.
- W1509651750 cites W1977391993 @default.
- W1509651750 cites W1978235730 @default.
- W1509651750 cites W1978815659 @default.
- W1509651750 cites W1979517124 @default.
- W1509651750 cites W1979614782 @default.
- W1509651750 cites W1980514772 @default.
- W1509651750 cites W1982072460 @default.
- W1509651750 cites W1982934014 @default.
- W1509651750 cites W1982943368 @default.
- W1509651750 cites W1983311772 @default.
- W1509651750 cites W1984984454 @default.
- W1509651750 cites W1985392546 @default.
- W1509651750 cites W1986144804 @default.
- W1509651750 cites W1987152869 @default.
- W1509651750 cites W1987533393 @default.
- W1509651750 cites W1987720655 @default.
- W1509651750 cites W1987906781 @default.
- W1509651750 cites W1988072486 @default.
- W1509651750 cites W1988343934 @default.
- W1509651750 cites W1988493810 @default.
- W1509651750 cites W1989050176 @default.
- W1509651750 cites W1989514904 @default.
- W1509651750 cites W1990859329 @default.
- W1509651750 cites W1991347106 @default.
- W1509651750 cites W1991794003 @default.
- W1509651750 cites W1992033325 @default.
- W1509651750 cites W1992062279 @default.
- W1509651750 cites W1992419032 @default.
- W1509651750 cites W1992767839 @default.
- W1509651750 cites W1992914135 @default.
- W1509651750 cites W1993074398 @default.
- W1509651750 cites W1993147282 @default.
- W1509651750 cites W1993970094 @default.
- W1509651750 cites W1994433056 @default.
- W1509651750 cites W1995366272 @default.