FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1509948560> ?p ?o ?g. }

• W1509948560 endingPage "4343" @default.
• W1509948560 startingPage "4331" @default.
• W1509948560 abstract "Rous sarcoma virus (RSV), a member of the avian sarcoma and leukosis family of retroviruses, has long been known to be capable of infecting and transforming mammalian cells; however, such transformed cells do not release virus particles. The RSV gag product (Pr76gag) produced in these cells is not released into the culture medium or proteolytically processed to release mature products. Thus, the behavior of Pr76gag in mammalian cells is much like that of mammalian retroviral Gag proteins which have been altered so as to block the addition of myristic acid at residue 2 (Gly). Because the RSV gag product does not possess a myristic acid addition site, we hypothesized that the creation of one by oligonucleotide-directed mutagenesis might permit particles to be released from mammalian cells. Two myristylated forms of Pr76 were created. In Pr76myr1, the first 10 amino acids have been exchanged for those of p60v-src, which are known to be sufficient for myristylation. In Pr76myr2, the Glu at the second residue has been substituted with Gly. The alleles encoding the modified and wild-type forms of Pr76 have been expressed at high levels in mammalian (CV-1) cells by using an SV40-based vector. Surprisingly, we have found that expression of high levels of the unmodified (wild-type) product, Pr76myr0, results in low levels of particle formation and precursor processing. This indicates that myristic acid is not the sole determinant for targeting. However, the addition of myristic acid to Pr76myr1 or Pr76myr2 resulted in a fivefold enhancement in Gag function. In all aspects examined, the behavior of myristylated Pr76 was identical to that of the authentic product produced in avian cells. We also show that processing is mediated by the gag-encoded protease and that removal of the amino terminus to create Pr76gagX results in an inability to form particles or be processed. This suggests that proper targeting is prerequisite for activation of the RSV protease in mammalian cells." @default.
• W1509948560 created "2016-06-24" @default.
• W1509948560 creator A5024391758 @default.
• W1509948560 creator A5055957785 @default.
• W1509948560 creator A5058181110 @default.
• W1509948560 date "1989-10-01" @default.
• W1509948560 modified "2023-10-11" @default.
• W1509948560 title "Creation and expression of myristylated forms of Rous sarcoma virus gag protein in mammalian cells" @default.
• W1509948560 cites W1493165726 @default.
• W1509948560 cites W1495973481 @default.
• W1509948560 cites W1496336749 @default.
• W1509948560 cites W1528763634 @default.
• W1509948560 cites W1534821110 @default.
• W1509948560 cites W1575815180 @default.
• W1509948560 cites W1591869598 @default.
• W1509948560 cites W1623171066 @default.
• W1509948560 cites W1854458012 @default.
• W1509948560 cites W1895421371 @default.
• W1509948560 cites W1912540402 @default.
• W1509948560 cites W1919290740 @default.
• W1509948560 cites W1928593456 @default.
• W1509948560 cites W1939482560 @default.
• W1509948560 cites W1969705974 @default.
• W1509948560 cites W1984279577 @default.
• W1509948560 cites W1986248827 @default.
• W1509948560 cites W1996834128 @default.
• W1509948560 cites W2014027374 @default.
• W1509948560 cites W2014588192 @default.
• W1509948560 cites W2016388741 @default.
• W1509948560 cites W2016760730 @default.
• W1509948560 cites W2021641297 @default.
• W1509948560 cites W2030202169 @default.
• W1509948560 cites W2038287032 @default.
• W1509948560 cites W2040843640 @default.
• W1509948560 cites W2052192499 @default.
• W1509948560 cites W2052919376 @default.
• W1509948560 cites W2057932255 @default.
• W1509948560 cites W2069117268 @default.
• W1509948560 cites W2076243780 @default.
• W1509948560 cites W2081812306 @default.
• W1509948560 cites W2083657154 @default.
• W1509948560 cites W2084193227 @default.
• W1509948560 cites W2088167128 @default.
• W1509948560 cites W2099434032 @default.
• W1509948560 cites W2101685255 @default.
• W1509948560 cites W2132095445 @default.
• W1509948560 cites W2135380694 @default.
• W1509948560 cites W2138270253 @default.
• W1509948560 cites W2153377782 @default.
• W1509948560 cites W2155329533 @default.
• W1509948560 cites W2160809286 @default.
• W1509948560 cites W2162692258 @default.
• W1509948560 cites W2288402731 @default.
• W1509948560 cites W2888511473 @default.
• W1509948560 cites W4231484789 @default.
• W1509948560 doi "https://doi.org/10.1128/jvi.63.10.4331-4343.1989" @default.
• W1509948560 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/251050" @default.
• W1509948560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2550669" @default.
• W1509948560 hasPublicationYear "1989" @default.
• W1509948560 type Work @default.
• W1509948560 sameAs 1509948560 @default.
• W1509948560 citedByCount "150" @default.
• W1509948560 countsByYear W15099485602012 @default.
• W1509948560 countsByYear W15099485602014 @default.
• W1509948560 countsByYear W15099485602015 @default.
• W1509948560 countsByYear W15099485602017 @default.
• W1509948560 countsByYear W15099485602018 @default.
• W1509948560 countsByYear W15099485602019 @default.
• W1509948560 countsByYear W15099485602020 @default.
• W1509948560 countsByYear W15099485602023 @default.
• W1509948560 crossrefType "journal-article" @default.
• W1509948560 hasAuthorship W1509948560A5024391758 @default.
• W1509948560 hasAuthorship W1509948560A5055957785 @default.
• W1509948560 hasAuthorship W1509948560A5058181110 @default.
• W1509948560 hasBestOaLocation W15099485601 @default.
• W1509948560 hasConcept C104317684 @default.
• W1509948560 hasConcept C143065580 @default.
• W1509948560 hasConcept C153911025 @default.
• W1509948560 hasConcept C159047783 @default.
• W1509948560 hasConcept C202878990 @default.
• W1509948560 hasConcept C2522874641 @default.
• W1509948560 hasConcept C2777441520 @default.
• W1509948560 hasConcept C2778483698 @default.
• W1509948560 hasConcept C2779936771 @default.
• W1509948560 hasConcept C515207424 @default.
• W1509948560 hasConcept C543025807 @default.
• W1509948560 hasConcept C55493867 @default.
• W1509948560 hasConcept C6881719 @default.
• W1509948560 hasConcept C86803240 @default.
• W1509948560 hasConceptScore W1509948560C104317684 @default.
• W1509948560 hasConceptScore W1509948560C143065580 @default.
• W1509948560 hasConceptScore W1509948560C153911025 @default.
• W1509948560 hasConceptScore W1509948560C159047783 @default.
• W1509948560 hasConceptScore W1509948560C202878990 @default.
• W1509948560 hasConceptScore W1509948560C2522874641 @default.
• W1509948560 hasConceptScore W1509948560C2777441520 @default.
• W1509948560 hasConceptScore W1509948560C2778483698 @default.
• W1509948560 hasConceptScore W1509948560C2779936771 @default.

• next