FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1510149907> ?p ?o ?g. }

• W1510149907 endingPage "670" @default.
• W1510149907 startingPage "663" @default.
• W1510149907 abstract "A 63-residue RNase A analog containing residues 26 to 35 then alanine, 41 to 59 and 73 to 84 then glycine, 100 to 110 then glycine, and 117 to 124 was synthesized by the solid phase method. The deletions comprised ordered (an alpha helix, parts of the beta sheet) and less ordered structures including 27 of the 56 residues invariant in mammalian ribonucleases. The synthetic 63-residue analog was cleaved from the insoluble support with liquid HF, reduced-reoxidized, fractionated by gel filtration, and purified further on an affinity column specific for the active site fold of RNase A. It had an activity of 8 to 14 per cent in the transphosphorylation step using poly(C) and poly(U) as substrates. It also had low synthetic and hydrolytic activity (0.2 per cent) and showed RNase A-like specificity toward the substrates tested. This indicated that all residues essential for substrate binding and catalysis were present and that their relative positions in the three-dimensional structure were probably very similar to those in native RNase A. Therefore, structure-function studies with the 63-residue RNase A analog should allow conclusions about the mode of action of the natural enzyme. As a first step in this direction, lysine 41 which is believed to be important for catalysis was replaced in the 63-residue analog by tyrosine or glutamine. The resulting (Tyr-41)- and (Gln 41)-63-residue analogs were also bound by the affinity column and had the same substrate specificity as native RNase A. They differed from each other, from the (Lys 41)-63-residue analog, and the 124-residue natural enzyme only with respect to the relative rates of the catalyzed reactions. Thus, lysine 41 does not seem to be essential for the functioning of RNase A." @default.
• W1510149907 created "2016-06-24" @default.
• W1510149907 creator A5078022995 @default.
• W1510149907 date "1977-01-01" @default.
• W1510149907 modified "2023-09-30" @default.
• W1510149907 title "Study of RNase A mechanism and folding by means of synthetic 63-residue analogs." @default.
• W1510149907 cites W1021151347 @default.
• W1510149907 cites W113688923 @default.
• W1510149907 cites W1505575303 @default.
• W1510149907 cites W1539795437 @default.
• W1510149907 cites W1556410937 @default.
• W1510149907 cites W1558346581 @default.
• W1510149907 cites W1590910500 @default.
• W1510149907 cites W1963519291 @default.
• W1510149907 cites W1963550288 @default.
• W1510149907 cites W1975161027 @default.
• W1510149907 cites W1979993560 @default.
• W1510149907 cites W2006370393 @default.
• W1510149907 cites W2013127846 @default.
• W1510149907 cites W2023140456 @default.
• W1510149907 cites W2025668897 @default.
• W1510149907 cites W2026352561 @default.
• W1510149907 cites W2027298627 @default.
• W1510149907 cites W2033860538 @default.
• W1510149907 cites W2043290803 @default.
• W1510149907 cites W2053952223 @default.
• W1510149907 cites W2054271515 @default.
• W1510149907 cites W2076067950 @default.
• W1510149907 cites W2148008715 @default.
• W1510149907 cites W2320556745 @default.
• W1510149907 cites W2334169772 @default.
• W1510149907 cites W2338888395 @default.
• W1510149907 cites W2396159587 @default.
• W1510149907 cites W2412306644 @default.
• W1510149907 cites W2468486354 @default.
• W1510149907 cites W2756557298 @default.
• W1510149907 cites W51337561 @default.
• W1510149907 cites W81755144 @default.
• W1510149907 doi "https://doi.org/10.1016/s0021-9258(17)32770-9" @default.
• W1510149907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/833149" @default.
• W1510149907 hasPublicationYear "1977" @default.
• W1510149907 type Work @default.
• W1510149907 sameAs 1510149907 @default.
• W1510149907 citedByCount "23" @default.
• W1510149907 countsByYear W15101499072017 @default.
• W1510149907 crossrefType "journal-article" @default.
• W1510149907 hasAuthorship W1510149907A5078022995 @default.
• W1510149907 hasBestOaLocation W15101499071 @default.
• W1510149907 hasConcept C104317684 @default.
• W1510149907 hasConcept C10879258 @default.
• W1510149907 hasConcept C119599485 @default.
• W1510149907 hasConcept C121332964 @default.
• W1510149907 hasConcept C12554922 @default.
• W1510149907 hasConcept C127413603 @default.
• W1510149907 hasConcept C185592680 @default.
• W1510149907 hasConcept C21951064 @default.
• W1510149907 hasConcept C2776545253 @default.
• W1510149907 hasConcept C2781338088 @default.
• W1510149907 hasConcept C55493867 @default.
• W1510149907 hasConcept C62520636 @default.
• W1510149907 hasConcept C67705224 @default.
• W1510149907 hasConcept C70721500 @default.
• W1510149907 hasConcept C71240020 @default.
• W1510149907 hasConcept C86803240 @default.
• W1510149907 hasConcept C89611455 @default.
• W1510149907 hasConceptScore W1510149907C104317684 @default.
• W1510149907 hasConceptScore W1510149907C10879258 @default.
• W1510149907 hasConceptScore W1510149907C119599485 @default.
• W1510149907 hasConceptScore W1510149907C121332964 @default.
• W1510149907 hasConceptScore W1510149907C12554922 @default.
• W1510149907 hasConceptScore W1510149907C127413603 @default.
• W1510149907 hasConceptScore W1510149907C185592680 @default.
• W1510149907 hasConceptScore W1510149907C21951064 @default.
• W1510149907 hasConceptScore W1510149907C2776545253 @default.
• W1510149907 hasConceptScore W1510149907C2781338088 @default.
• W1510149907 hasConceptScore W1510149907C55493867 @default.
• W1510149907 hasConceptScore W1510149907C62520636 @default.
• W1510149907 hasConceptScore W1510149907C67705224 @default.
• W1510149907 hasConceptScore W1510149907C70721500 @default.
• W1510149907 hasConceptScore W1510149907C71240020 @default.
• W1510149907 hasConceptScore W1510149907C86803240 @default.
• W1510149907 hasConceptScore W1510149907C89611455 @default.
• W1510149907 hasIssue "2" @default.
• W1510149907 hasLocation W15101499071 @default.
• W1510149907 hasOpenAccess W1510149907 @default.
• W1510149907 hasPrimaryLocation W15101499071 @default.
• W1510149907 hasRelatedWork W1493551487 @default.
• W1510149907 hasRelatedWork W1989778206 @default.
• W1510149907 hasRelatedWork W2011699091 @default.
• W1510149907 hasRelatedWork W2092756551 @default.
• W1510149907 hasRelatedWork W2151214460 @default.
• W1510149907 hasRelatedWork W2330215216 @default.
• W1510149907 hasRelatedWork W2377467777 @default.
• W1510149907 hasRelatedWork W2950269921 @default.
• W1510149907 hasRelatedWork W3157523411 @default.
• W1510149907 hasRelatedWork W4242230499 @default.
• W1510149907 hasVolume "252" @default.
• W1510149907 isParatext "false" @default.

• next