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- W1510367500 abstract "Significant IgM antibody responses to the pneumococcal capsular polysaccharide were demonstrated in nurse sharks immunized with pneumococcal vaccines. The antibodies isolated from immune sera by affinity chromatography were exclusively of the 19S variety; no 7S antibodies were isolated from any of six animals studied for periods up to twelve months. Equilibrium dialysis studies of the isolated antibodies demonstrated several important points: (1) the IgM antibodies contained ten combining sites per 19S molecule, (2) in all cases, the isolated antibodies showed marked heterogeneity of combining sites, (3) the antibodies were all of low average affinity, and (4) there was no increase in the average affinity of the antibodies isolated from any single animal for periods of up to twelve months after the start of immunization. In order to determine if a single IgM molecule contains ten equivalent combining sites, the antibodies isolated from several animals were fractionated by liquid isoelectric focusing. Equilibrium dialysis experiments using focused fractions showed the presence of ten functionally identical combining sites per IgM molecule. As a proof of the structural homogeneity of focused fractions, antibodies were separated into H and L chains, § recombined and tested for the regeneration of the original combining site by equilibrium dialysis. The results indicated that recombinants of focused antibody fractions contained binding sites identical to those of the intact antibody, whereas heterogeneous (unfocused) recombinants and isolated H and L chains failed to show any binding activity. The conclusion from this study is that the heterogeneity of ligand binding exhibited by nurse shark 19S antibodies to the capsular polysaccharide of the Type III pneumococcus can be attributed to intermolecular heterogeneity." @default.
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- W1510367500 date "1980-03-01" @default.
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- W1510367500 title "Phylogeny of immunoglobulin structure and function—VIII" @default.
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- W1510367500 doi "https://doi.org/10.1016/0161-5890(80)90057-7" @default.
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