FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1510583609> ?p ?o ?g. }

• W1510583609 endingPage "23233" @default.
• W1510583609 startingPage "23227" @default.
• W1510583609 abstract "To study transactivation by the Xenopus laevis estrogen receptor (XER), we inserted one or two copies of a synthetic amphipathic helix at amino acid 276 of the XER. The XER mutants containing one or two copies of the amphipathic helix (XER/1AH and XER/2AH, respectively) and wild-type XER were expressed at similar levels. In transient transfection assays, XER/1AH exhibited only a modest, promoter-specific increase in transactivation. Constitutive (estrogen-independent) transcription of a synthetic promoter containing two estrogen response elements (EREs) was approximately 10-fold higher for the XER/2AH mutant than for wild-type XER. The XER/2AH mutant and wild-type XER exhibited similar 17 beta-estradiol dose-response curves for transactivation. In studies carried out over a broad range of DNA concentrations using the simple 2ERE-TATA promoter or a complex vitellogenin-derived promoter, the XER/2AH mutant exhibited an estrogen-dependent 2-3-fold increase in transactivation. A 2-3-fold increase in transactivation by XER/2AH was also observed using synthetic promoters in which the two EREs exhibit synergistic interactions with the NF1, AP1, or vitellogenin activator upstream activator sequences. Using a promoter interference assay to investigate intracellular interactions between the estrogen receptor and the ERE, we showed that binding of wild-type XER to the ERE was strongly estrogen-dependent. In the presence of 17 beta-estradiol, XER/2AH and wild-type XER exhibited similar promoter interference curves. In the absence of 17 beta-estradiol, the expression plasmid encoding the XER/2AH mutant achieved levels of promoter interference with 0.25-0.5 microgram of transfected DNA that were similar to those observed with 5-10 micrograms of the expression plasmid encoding wild-type XER. The ability of the XER/2AH mutant to activate transcription in the absence of estrogen therefore is likely to be related to the approximately 20-fold increase in its apparent ability to bind to the ERE. Since XER/2AH was unable to activate transcription from a glucocorticoid response element, enhanced binding of XER/2AH to the ERE did not result from a general increase in binding to DNA. The XER/2AH mutant appears to be the first nuclear receptor mutant to retain hormone-dependent transactivation and to exhibit enhanced hormone-independent binding to its hormone response element." @default.
• W1510583609 created "2016-06-24" @default.
• W1510583609 creator A5061599949 @default.
• W1510583609 creator A5087725251 @default.
• W1510583609 date "1993-11-01" @default.
• W1510583609 modified "2023-10-15" @default.
• W1510583609 title "An estrogen receptor mutant exhibiting hormone-independent transactivation and enhanced affinity for the estrogen response element." @default.
• W1510583609 cites W1489022720 @default.
• W1510583609 cites W1493638 @default.
• W1510583609 cites W1506602202 @default.
• W1510583609 cites W1510421758 @default.
• W1510583609 cites W1534882737 @default.
• W1510583609 cites W1556861001 @default.
• W1510583609 cites W1571431043 @default.
• W1510583609 cites W1585603261 @default.
• W1510583609 cites W1591964692 @default.
• W1510583609 cites W1723187988 @default.
• W1510583609 cites W1964520022 @default.
• W1510583609 cites W1967013096 @default.
• W1510583609 cites W1971960644 @default.
• W1510583609 cites W1975715610 @default.
• W1510583609 cites W1977538475 @default.
• W1510583609 cites W1979029637 @default.
• W1510583609 cites W1981771606 @default.
• W1510583609 cites W2005914718 @default.
• W1510583609 cites W2006729630 @default.
• W1510583609 cites W2007587099 @default.
• W1510583609 cites W2010309484 @default.
• W1510583609 cites W2014107736 @default.
• W1510583609 cites W2019673113 @default.
• W1510583609 cites W2029475329 @default.
• W1510583609 cites W2037705033 @default.
• W1510583609 cites W2055354691 @default.
• W1510583609 cites W2060903877 @default.
• W1510583609 cites W2066441904 @default.
• W1510583609 cites W2067598960 @default.
• W1510583609 cites W2068577315 @default.
• W1510583609 cites W2079110550 @default.
• W1510583609 cites W2085124463 @default.
• W1510583609 cites W2105014300 @default.
• W1510583609 cites W2121625670 @default.
• W1510583609 cites W2129972061 @default.
• W1510583609 cites W2134399858 @default.
• W1510583609 cites W2153550326 @default.
• W1510583609 doi "https://doi.org/10.1016/s0021-9258(19)49453-2" @default.
• W1510583609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8226845" @default.
• W1510583609 hasPublicationYear "1993" @default.
• W1510583609 type Work @default.
• W1510583609 sameAs 1510583609 @default.
• W1510583609 citedByCount "15" @default.
• W1510583609 crossrefType "journal-article" @default.
• W1510583609 hasAuthorship W1510583609A5061599949 @default.
• W1510583609 hasAuthorship W1510583609A5087725251 @default.
• W1510583609 hasBestOaLocation W15105836091 @default.
• W1510583609 hasConcept C101762097 @default.
• W1510583609 hasConcept C104317684 @default.
• W1510583609 hasConcept C121608353 @default.
• W1510583609 hasConcept C126322002 @default.
• W1510583609 hasConcept C1292079 @default.
• W1510583609 hasConcept C132376346 @default.
• W1510583609 hasConcept C134018914 @default.
• W1510583609 hasConcept C143065580 @default.
• W1510583609 hasConcept C150194340 @default.
• W1510583609 hasConcept C170493617 @default.
• W1510583609 hasConcept C185592680 @default.
• W1510583609 hasConcept C2777164284 @default.
• W1510583609 hasConcept C502942594 @default.
• W1510583609 hasConcept C51445715 @default.
• W1510583609 hasConcept C530470458 @default.
• W1510583609 hasConcept C55493867 @default.
• W1510583609 hasConcept C71924100 @default.
• W1510583609 hasConcept C84606932 @default.
• W1510583609 hasConcept C86339819 @default.
• W1510583609 hasConcept C86803240 @default.
• W1510583609 hasConcept C96307122 @default.
• W1510583609 hasConceptScore W1510583609C101762097 @default.
• W1510583609 hasConceptScore W1510583609C104317684 @default.
• W1510583609 hasConceptScore W1510583609C121608353 @default.
• W1510583609 hasConceptScore W1510583609C126322002 @default.
• W1510583609 hasConceptScore W1510583609C1292079 @default.
• W1510583609 hasConceptScore W1510583609C132376346 @default.
• W1510583609 hasConceptScore W1510583609C134018914 @default.
• W1510583609 hasConceptScore W1510583609C143065580 @default.
• W1510583609 hasConceptScore W1510583609C150194340 @default.
• W1510583609 hasConceptScore W1510583609C170493617 @default.
• W1510583609 hasConceptScore W1510583609C185592680 @default.
• W1510583609 hasConceptScore W1510583609C2777164284 @default.
• W1510583609 hasConceptScore W1510583609C502942594 @default.
• W1510583609 hasConceptScore W1510583609C51445715 @default.
• W1510583609 hasConceptScore W1510583609C530470458 @default.
• W1510583609 hasConceptScore W1510583609C55493867 @default.
• W1510583609 hasConceptScore W1510583609C71924100 @default.
• W1510583609 hasConceptScore W1510583609C84606932 @default.
• W1510583609 hasConceptScore W1510583609C86339819 @default.
• W1510583609 hasConceptScore W1510583609C86803240 @default.
• W1510583609 hasConceptScore W1510583609C96307122 @default.
• W1510583609 hasIssue "31" @default.
• W1510583609 hasLocation W15105836091 @default.

• next