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• W1510621398 abstract "Abstract The ability to visualize in real time the expression dynamics and localization of specific RNAs in vivo offers tremendous opportunities for biological and disease studies, including the detection of cancer. Quantitative methods such as real‐time PCR and DNA microarrays rely on the use of cell lysates, and thus are not able to obtain important spatial and temporal information. Fluorescent proteins and other reporter systems cannot image endogenous RNA in living cells. Fluorescence in situ hybridization (FISH) assays require washing to achieve specificity, and therefore can only be used with fixed cells. In this chapter we review the recent development of nanostructured probes for living cell RNA detection, and discuss the biological and engineering issues and challenges of quantifying gene expression in vivo . In particular, we describe methods that use oligonucleotide probes, combined with novel delivery strategies, to image the relative level, localization and dynamics of RNA in live cells. Examples of detecting endogenous mRNAs, as well as imaging their subcellular localization, are given to illustrate the biological applications. Issues in probe design, delivery and target accessibility are also discussed. The nanostructured probes promise to open new and exciting opportunities in sensitive gene detection for a wide range of biological and medical applications." @default.
• W1510621398 created "2016-06-24" @default.
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• W1510621398 date "2010-03-15" @default.
• W1510621398 modified "2023-10-11" @default.
• W1510621398 title "Nanostructured Probes for <i>In Vivo</i> Gene Detection" @default.
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• W1510621398 doi "https://doi.org/10.1002/9783527628155.nanotech054" @default.
• W1510621398 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3226718" @default.
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