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- W1510698102 abstract "A procedure to purify to homogeneity the active form as well as the proenzyme form of the acidic protease of human seminal plasma is described. This involved precipitation with ammonium sulfate, chromatography on diethylaminoethylcellulose, Sephadex G-200, and Sephadex G-100. The molecular weights of the active form and of the proenzyme were determined by electrophoresis and gel filtration to be 35,000 and 42,000, respectively. The proenzyme was more stable than the active form in alkaline solution and can be converted into the active enzyme under acidic conditions. The active form of the acidic protease can hydrolyze hemoglobin, N,N'-dimethylcasein, N-acetyl-L-phenylalanyl-L-diiodotyrosine, and N-benzyloxycarbonyl-L-glutamyl-L-phenylalanine, but cannot hydrolyze bovine serum albumin, ovalbumin, N-benzyloxycarbonyl-L-glutamyl-L-tyrosine. The active form was also inhibited by p-bromophenacyl bromide and 1,2-epoxy-3-(p-nitrophenoxy)propane." @default.
- W1510698102 created "2016-06-24" @default.
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- W1510698102 date "1975-10-01" @default.
- W1510698102 modified "2023-09-26" @default.
- W1510698102 title "Acidic protease from human seminal plasma. Purification and some properties of active enzyme and of proenzyme." @default.
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- W1510698102 doi "https://doi.org/10.1016/s0021-9258(19)40856-9" @default.
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