Matches in SemOpenAlex for { <https://semopenalex.org/work/W1511013129> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1511013129 endingPage "4268" @default.
- W1511013129 startingPage "4260" @default.
- W1511013129 abstract "The electron-carrying cofactor NADP is formed by phosphorylation of NAD. A strategy for the isolation of NAD kinase mutants revealed two classes of temperature-sensitive mutations, nadF and nadG, mapping at min 13 and 72 of the Salmonella chromosome. Both mutant types grew on nutrient broth at both 30 and 42 degrees C but on minimal medium showed a temperature-sensitive growth defect which was not corrected by any of the single nutritional supplements tested. A nadF deletion mutant grew on nutrient broth but not on minimal medium. A double mutant with the nadF deletion and a nadG(Ts) mutation showed temperature-sensitive growth on all media. We propose that Salmonella typhimurium has two NAD kinases, one encoded by the nadF and one by the nadG gene. This is supported by the fact that temperature-sensitive mutants of both genes produce kinase activity with altered heat stability. Results suggest that either one of two NAD kinases is sufficient for growth on rich medium, but that both are needed for growth on minimal media. Enzyme assays show that the nadF gene is responsible for about 70% of total NAD kinase activity, and that the nadG gene dictates the remaining 30%. While testing nutritional phenotypes of nadF and nadG mutants, we found that the biosynthetic intermediate, quinolinic acid (QA) inhibited growth of nadF mutants on nutrient broth. This suggested that the NadG enzyme might be inhibited by QA. Enzyme assays demonstrated that QA inhibits the NadG but not the NadF enzyme. This suggests the existence of a regulatory mechanism which controls NADP levels." @default.
- W1511013129 created "2016-06-24" @default.
- W1511013129 creator A5019506751 @default.
- W1511013129 creator A5062133521 @default.
- W1511013129 date "1994-07-01" @default.
- W1511013129 modified "2023-09-23" @default.
- W1511013129 title "Evidence for two NAD kinases in Salmonella typhimurium" @default.
- W1511013129 cites W1022642962 @default.
- W1511013129 cites W1023150109 @default.
- W1511013129 cites W1484292938 @default.
- W1511013129 cites W1486946238 @default.
- W1511013129 cites W1507231322 @default.
- W1511013129 cites W1524570212 @default.
- W1511013129 cites W1558547246 @default.
- W1511013129 cites W1576074336 @default.
- W1511013129 cites W1775749144 @default.
- W1511013129 cites W1804011389 @default.
- W1511013129 cites W181908322 @default.
- W1511013129 cites W1829598675 @default.
- W1511013129 cites W185104107 @default.
- W1511013129 cites W1906848714 @default.
- W1511013129 cites W1970408872 @default.
- W1511013129 cites W1972671967 @default.
- W1511013129 cites W2028585334 @default.
- W1511013129 cites W2040388799 @default.
- W1511013129 cites W2047560791 @default.
- W1511013129 cites W2059371275 @default.
- W1511013129 cites W2069333966 @default.
- W1511013129 cites W2103783269 @default.
- W1511013129 cites W2104049345 @default.
- W1511013129 cites W2109263179 @default.
- W1511013129 cites W2143847453 @default.
- W1511013129 cites W2154936481 @default.
- W1511013129 cites W2322695924 @default.
- W1511013129 doi "https://doi.org/10.1128/jb.176.14.4260-4268.1994" @default.
- W1511013129 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/205637" @default.
- W1511013129 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8021211" @default.
- W1511013129 hasPublicationYear "1994" @default.
- W1511013129 type Work @default.
- W1511013129 sameAs 1511013129 @default.
- W1511013129 citedByCount "17" @default.
- W1511013129 countsByYear W15110131292013 @default.
- W1511013129 countsByYear W15110131292018 @default.
- W1511013129 crossrefType "journal-article" @default.
- W1511013129 hasAuthorship W1511013129A5019506751 @default.
- W1511013129 hasAuthorship W1511013129A5062133521 @default.
- W1511013129 hasBestOaLocation W15110131292 @default.
- W1511013129 hasConcept C104317684 @default.
- W1511013129 hasConcept C143065580 @default.
- W1511013129 hasConcept C153911025 @default.
- W1511013129 hasConcept C181199279 @default.
- W1511013129 hasConcept C184235292 @default.
- W1511013129 hasConcept C197957613 @default.
- W1511013129 hasConcept C55493867 @default.
- W1511013129 hasConcept C75520062 @default.
- W1511013129 hasConcept C86803240 @default.
- W1511013129 hasConceptScore W1511013129C104317684 @default.
- W1511013129 hasConceptScore W1511013129C143065580 @default.
- W1511013129 hasConceptScore W1511013129C153911025 @default.
- W1511013129 hasConceptScore W1511013129C181199279 @default.
- W1511013129 hasConceptScore W1511013129C184235292 @default.
- W1511013129 hasConceptScore W1511013129C197957613 @default.
- W1511013129 hasConceptScore W1511013129C55493867 @default.
- W1511013129 hasConceptScore W1511013129C75520062 @default.
- W1511013129 hasConceptScore W1511013129C86803240 @default.
- W1511013129 hasIssue "14" @default.
- W1511013129 hasLocation W15110131291 @default.
- W1511013129 hasLocation W15110131292 @default.
- W1511013129 hasLocation W15110131293 @default.
- W1511013129 hasLocation W15110131294 @default.
- W1511013129 hasOpenAccess W1511013129 @default.
- W1511013129 hasPrimaryLocation W15110131291 @default.
- W1511013129 hasRelatedWork W1953994264 @default.
- W1511013129 hasRelatedWork W2053807978 @default.
- W1511013129 hasRelatedWork W2113094549 @default.
- W1511013129 hasRelatedWork W2118026715 @default.
- W1511013129 hasRelatedWork W2169273154 @default.
- W1511013129 hasRelatedWork W2277076226 @default.
- W1511013129 hasRelatedWork W2596200008 @default.
- W1511013129 hasRelatedWork W2766457803 @default.
- W1511013129 hasRelatedWork W2972189026 @default.
- W1511013129 hasRelatedWork W4229372104 @default.
- W1511013129 hasVolume "176" @default.
- W1511013129 isParatext "false" @default.
- W1511013129 isRetracted "false" @default.
- W1511013129 magId "1511013129" @default.
- W1511013129 workType "article" @default.