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- W1511087419 abstract "The site of synthesis of 1,2-disaturated-(diacyl)-sn-glycero-3-phosphocholine (Sat2PC) in mouse alveolar type II cell adenomas has been studied by conducting pulse-chase experiments. Isolation of microsomal and lamellar body fractions from adenomas after a 20-min pulse with [methyl-3H]choline demonstrates that Sat2PC first appears in the microsomal fraction, and after a short lag subsequently appears in the lamellar body fraction. The kinetics of labeling of Sat2PC are consistent with the microsomal membranes functioning as the subcellular site of synthesis for this pulmonary surfactant phospholipid. Short term labeling experiments with [9,10-3H]palmitate demonstrate that this fatty acid is incorporated into the sn-2 position of Sat2PC at a faster rate than its incorporation into the sn-1 position. This finding indicates that the synthesis of Sat2PC occurs by a deacylation-reacylation mechanism." @default.
- W1511087419 created "2016-06-24" @default.
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- W1511087419 date "1979-10-01" @default.
- W1511087419 modified "2023-10-01" @default.
- W1511087419 title "Subcellular site and mechanism of synthesis of disaturated phosphatidylcholine in alveolar type II cell adenomas." @default.
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- W1511087419 doi "https://doi.org/10.1016/s0021-9258(19)86938-7" @default.
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