Matches in SemOpenAlex for { <https://semopenalex.org/work/W1511830885> ?p ?o ?g. }
- W1511830885 endingPage "1131" @default.
- W1511830885 startingPage "1125" @default.
- W1511830885 abstract "Serine phosphorylation of insulin-like growth factor binding protein-1 (IGFBP-1) has been shown to alter its affinity for the insulin-like growth factors (IGF-I and IGF-II) and to modify its capacity to modulate cellular responses to the IGFs. Because of this, we determined the sites of serine phosphorylation. Purification of 32P-labeled IGFBP-1 was followed by digestion with trypsin and endoproteinase Glu-C and radiosequencing of labeled peptides. Three serines were found to be phosphorylated, with Ser101, Ser119, and Ser169 containing 70%, 5%, and 25% of the incorporated 32P, respectively. A mutated IGFBP-1, substituting alanine for serine at positions 98 and 101, was expressed in CHO cells. On nondenaturing gels, the wild type protein migrated as five isoforms (one non-phosphorylated and four phosphorylated). However, in the mutated protein, the most rapidly migrating band (a phosphorylated form) was not present. The cells containing the mutated cDNA incorporated 60% less 32P into immunoprecipitable IGFBP-1. The mutated protein had a 3-fold reduction in affinity for IGF-I compared to the wild type protein. We conclude that Ser101 represents the major site of phosphorylation containing 63% of the total 32P incorporated and that phosphorylation of Ser101 is important for maintenance of high affinity binding for this growth factor." @default.
- W1511830885 created "2016-06-24" @default.
- W1511830885 creator A5000288481 @default.
- W1511830885 creator A5002944373 @default.
- W1511830885 creator A5030816003 @default.
- W1511830885 creator A5039368198 @default.
- W1511830885 creator A5047854064 @default.
- W1511830885 creator A5091418550 @default.
- W1511830885 date "1993-01-01" @default.
- W1511830885 modified "2023-10-15" @default.
- W1511830885 title "Identification of the sites of phosphorylation in insulin-like growth factor binding protein-1. Regulation of its affinity by phosphorylation of serine 101." @default.
- W1511830885 cites W1501164720 @default.
- W1511830885 cites W1540104874 @default.
- W1511830885 cites W1571404318 @default.
- W1511830885 cites W1602528313 @default.
- W1511830885 cites W1605635614 @default.
- W1511830885 cites W1850214287 @default.
- W1511830885 cites W2006156624 @default.
- W1511830885 cites W2019331142 @default.
- W1511830885 cites W2022961230 @default.
- W1511830885 cites W2032558447 @default.
- W1511830885 cites W2049749719 @default.
- W1511830885 cites W2082201342 @default.
- W1511830885 cites W2083406280 @default.
- W1511830885 cites W2084310213 @default.
- W1511830885 cites W2090099477 @default.
- W1511830885 cites W2111666458 @default.
- W1511830885 cites W2130472874 @default.
- W1511830885 cites W2138270253 @default.
- W1511830885 doi "https://doi.org/10.1016/s0021-9258(18)54050-3" @default.
- W1511830885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7678248" @default.
- W1511830885 hasPublicationYear "1993" @default.
- W1511830885 type Work @default.
- W1511830885 sameAs 1511830885 @default.
- W1511830885 citedByCount "82" @default.
- W1511830885 countsByYear W15118308852012 @default.
- W1511830885 countsByYear W15118308852013 @default.
- W1511830885 countsByYear W15118308852015 @default.
- W1511830885 countsByYear W15118308852016 @default.
- W1511830885 countsByYear W15118308852017 @default.
- W1511830885 countsByYear W15118308852018 @default.
- W1511830885 countsByYear W15118308852020 @default.
- W1511830885 countsByYear W15118308852021 @default.
- W1511830885 countsByYear W15118308852022 @default.
- W1511830885 countsByYear W15118308852023 @default.
- W1511830885 crossrefType "journal-article" @default.
- W1511830885 hasAuthorship W1511830885A5000288481 @default.
- W1511830885 hasAuthorship W1511830885A5002944373 @default.
- W1511830885 hasAuthorship W1511830885A5030816003 @default.
- W1511830885 hasAuthorship W1511830885A5039368198 @default.
- W1511830885 hasAuthorship W1511830885A5047854064 @default.
- W1511830885 hasAuthorship W1511830885A5091418550 @default.
- W1511830885 hasBestOaLocation W15118308851 @default.
- W1511830885 hasConcept C116834253 @default.
- W1511830885 hasConcept C11960822 @default.
- W1511830885 hasConcept C170493617 @default.
- W1511830885 hasConcept C185592680 @default.
- W1511830885 hasConcept C2775960820 @default.
- W1511830885 hasConcept C2776414213 @default.
- W1511830885 hasConcept C2780378035 @default.
- W1511830885 hasConcept C2780689927 @default.
- W1511830885 hasConcept C55493867 @default.
- W1511830885 hasConcept C59822182 @default.
- W1511830885 hasConcept C86803240 @default.
- W1511830885 hasConcept C87325107 @default.
- W1511830885 hasConcept C95444343 @default.
- W1511830885 hasConcept C97029542 @default.
- W1511830885 hasConceptScore W1511830885C116834253 @default.
- W1511830885 hasConceptScore W1511830885C11960822 @default.
- W1511830885 hasConceptScore W1511830885C170493617 @default.
- W1511830885 hasConceptScore W1511830885C185592680 @default.
- W1511830885 hasConceptScore W1511830885C2775960820 @default.
- W1511830885 hasConceptScore W1511830885C2776414213 @default.
- W1511830885 hasConceptScore W1511830885C2780378035 @default.
- W1511830885 hasConceptScore W1511830885C2780689927 @default.
- W1511830885 hasConceptScore W1511830885C55493867 @default.
- W1511830885 hasConceptScore W1511830885C59822182 @default.
- W1511830885 hasConceptScore W1511830885C86803240 @default.
- W1511830885 hasConceptScore W1511830885C87325107 @default.
- W1511830885 hasConceptScore W1511830885C95444343 @default.
- W1511830885 hasConceptScore W1511830885C97029542 @default.
- W1511830885 hasIssue "2" @default.
- W1511830885 hasLocation W15118308851 @default.
- W1511830885 hasLocation W15118308852 @default.
- W1511830885 hasOpenAccess W1511830885 @default.
- W1511830885 hasPrimaryLocation W15118308851 @default.
- W1511830885 hasRelatedWork W1994750899 @default.
- W1511830885 hasRelatedWork W2014231481 @default.
- W1511830885 hasRelatedWork W2035731217 @default.
- W1511830885 hasRelatedWork W2049777033 @default.
- W1511830885 hasRelatedWork W2057779043 @default.
- W1511830885 hasRelatedWork W2121592873 @default.
- W1511830885 hasRelatedWork W2130733926 @default.
- W1511830885 hasRelatedWork W2439036626 @default.
- W1511830885 hasRelatedWork W2912581221 @default.
- W1511830885 hasRelatedWork W2189320406 @default.
- W1511830885 hasVolume "268" @default.
- W1511830885 isParatext "false" @default.