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- W1512903761 abstract "Publisher Summary In studies of subunit structure, equivalent weight and mechanism of action of enzymes, an accurate measure of the weight-concentration of the highly purified protein in solution is required. Concentration is usually measured spectrophotometrically, utilizing chromophores associated with the protein itself or chromophores produced in the Folin–Lowry or biuret procedures. In any spectrophotometric assay of protein concentration, it is necessary to know the relationship between the weight-concentration of the protein and the absorbance measured. The chapter describes an absolute gravimetric method for determining the weight-concentration of a protein solution. This method is useful in standardizing the measurement of concentration of a pure protein by any spectrophotometric method. One of the most important physical characteristics of an enzyme is its specific activity. Because of the convenience of measuring the concentration of protein by means of its A 280 , it is suggested that, where possible, the specific activity of an enzyme be expressed in terms of the empirical A 280 rather than in terms of milligram per milliliter or milligram of protein." @default.
- W1512903761 created "2016-06-24" @default.
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- W1512903761 date "1975-01-01" @default.
- W1512903761 modified "2023-10-14" @default.
- W1512903761 title "[26] Appendix: An accurate gravimetric determination of the concentration of pure proteins and the specific activity of enzymes" @default.
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- W1512903761 doi "https://doi.org/10.1016/0076-6879(75)35157-4" @default.
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