FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1513132009> ?p ?o ?g. }

• W1513132009 endingPage "120" @default.
• W1513132009 startingPage "107" @default.
• W1513132009 abstract "Cells of Bacillus subtilis can enter a natural physiological state, termed competence, that is permissive for uptake of DNA from the surrounding medium. In the B. subtilis genetic system, transfection refers to uptake of isolated bacteriophage DNA by competent host cells, followed by intracellular processing that may ultimately lead to productive infection. Previous investigations have shown that transfecting DNA is usually far less infectious (on a molar basis) than is the DNA injected by phage particles; this result is apparently due to inactivating events suffered by transfecting DNA during its metabolism by competent cells. Earlier studies also demonstrated that, in some cases, the infectivity of transfecting DNA can be increased by ultraviolet (UV) irradiation of the competent cells prior to transfection, or by cotransfection of UV-irradiated heterologous DNAs; collectively, these phenomena have been termed transfection enhancement (TE). We propose here that some transfecting B. subtilis phage DNAs are attacked by a novel host DNA repair system, and that TE reflects inhibition of this by a competing substrate in UV-irradiated DNA. In support of this model, we show that UV-DNA cotransfection leads to a reduced rate of intracellular endonucleolytic breakdown of transfecting DNA. We also demonstrate that TE displays marked specificity of a kind frequently observed for repair enzymes. Thus, phages that contain hydroxymethyl uracil (HMU), but not thymine, in their genomes are susceptible to this process. In addition, we show that the photoproduct(s) in UV-irradiated DNA that produces TE by cotransfection is specific, and is not uracil, a pyrimidine dimer, thymine glycol, HMU, or a substrate for the E. coli thymine glycol DNA N-glycosylase. This photoproduct is derivable from thymine or HMU. The implications of these results are discussed." @default.
• W1513132009 created "2016-06-24" @default.
• W1513132009 creator A5021451982 @default.
• W1513132009 creator A5030321272 @default.
• W1513132009 creator A5041057852 @default.
• W1513132009 creator A5044862124 @default.
• W1513132009 creator A5045067253 @default.
• W1513132009 date "1997-08-01" @default.
• W1513132009 modified "2023-09-24" @default.
• W1513132009 title "Transfection enhancement in Bacillus subtilis displays features of a novel DNA repair pathway" @default.
• W1513132009 cites W135588916 @default.
• W1513132009 cites W14824223 @default.
• W1513132009 cites W1500049033 @default.
• W1513132009 cites W1526143887 @default.
• W1513132009 cites W1531374249 @default.
• W1513132009 cites W1534252069 @default.
• W1513132009 cites W1551043917 @default.
• W1513132009 cites W1553505461 @default.
• W1513132009 cites W1563525014 @default.
• W1513132009 cites W1579004914 @default.
• W1513132009 cites W1589015308 @default.
• W1513132009 cites W1599680743 @default.
• W1513132009 cites W1601733367 @default.
• W1513132009 cites W1614195876 @default.
• W1513132009 cites W1676315537 @default.
• W1513132009 cites W1756860854 @default.
• W1513132009 cites W1893007007 @default.
• W1513132009 cites W1940668749 @default.
• W1513132009 cites W1956214299 @default.
• W1513132009 cites W1975177743 @default.
• W1513132009 cites W1984359964 @default.
• W1513132009 cites W1988230291 @default.
• W1513132009 cites W1992584287 @default.
• W1513132009 cites W1998649277 @default.
• W1513132009 cites W2011389780 @default.
• W1513132009 cites W2011449534 @default.
• W1513132009 cites W2011634180 @default.
• W1513132009 cites W2012286136 @default.
• W1513132009 cites W2031414855 @default.
• W1513132009 cites W2032355188 @default.
• W1513132009 cites W2034129646 @default.
• W1513132009 cites W2035496096 @default.
• W1513132009 cites W2038787486 @default.
• W1513132009 cites W2042980947 @default.
• W1513132009 cites W2054551897 @default.
• W1513132009 cites W2055456220 @default.
• W1513132009 cites W2057321426 @default.
• W1513132009 cites W2059641675 @default.
• W1513132009 cites W2060822442 @default.
• W1513132009 cites W2075236139 @default.
• W1513132009 cites W2075459946 @default.
• W1513132009 cites W2090436561 @default.
• W1513132009 cites W2090849357 @default.
• W1513132009 cites W2091811251 @default.
• W1513132009 cites W2099789574 @default.
• W1513132009 cites W2115466238 @default.
• W1513132009 cites W2120561953 @default.
• W1513132009 cites W2138434854 @default.
• W1513132009 cites W2152531997 @default.
• W1513132009 cites W2165559376 @default.
• W1513132009 cites W2325632843 @default.
• W1513132009 cites W4247994150 @default.
• W1513132009 doi "https://doi.org/10.1016/s0921-8777(97)00019-0" @default.
• W1513132009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9298119" @default.
• W1513132009 hasPublicationYear "1997" @default.
• W1513132009 type Work @default.
• W1513132009 sameAs 1513132009 @default.
• W1513132009 citedByCount "1" @default.
• W1513132009 crossrefType "journal-article" @default.
• W1513132009 hasAuthorship W1513132009A5021451982 @default.
• W1513132009 hasAuthorship W1513132009A5030321272 @default.
• W1513132009 hasAuthorship W1513132009A5041057852 @default.
• W1513132009 hasAuthorship W1513132009A5044862124 @default.
• W1513132009 hasAuthorship W1513132009A5045067253 @default.
• W1513132009 hasConcept C104317684 @default.
• W1513132009 hasConcept C134935766 @default.
• W1513132009 hasConcept C153911025 @default.
• W1513132009 hasConcept C167975912 @default.
• W1513132009 hasConcept C182420341 @default.
• W1513132009 hasConcept C2776858809 @default.
• W1513132009 hasConcept C2777272437 @default.
• W1513132009 hasConcept C523546767 @default.
• W1513132009 hasConcept C54009773 @default.
• W1513132009 hasConcept C54355233 @default.
• W1513132009 hasConcept C552990157 @default.
• W1513132009 hasConcept C55493867 @default.
• W1513132009 hasConcept C86803240 @default.
• W1513132009 hasConcept C95444343 @default.
• W1513132009 hasConceptScore W1513132009C104317684 @default.
• W1513132009 hasConceptScore W1513132009C134935766 @default.
• W1513132009 hasConceptScore W1513132009C153911025 @default.
• W1513132009 hasConceptScore W1513132009C167975912 @default.
• W1513132009 hasConceptScore W1513132009C182420341 @default.
• W1513132009 hasConceptScore W1513132009C2776858809 @default.
• W1513132009 hasConceptScore W1513132009C2777272437 @default.
• W1513132009 hasConceptScore W1513132009C523546767 @default.
• W1513132009 hasConceptScore W1513132009C54009773 @default.
• W1513132009 hasConceptScore W1513132009C54355233 @default.

• next