FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1513314547> ?p ?o ?g. }

• W1513314547 endingPage "32" @default.
• W1513314547 startingPage "22" @default.
• W1513314547 abstract "Apart from genetic mutations, epigenetic alteration is a common phenomenon that contributes to neoplastic transformation in colorectal cancer. Transcriptional silencing of tumor-suppressor genes without changes in the DNA sequence is explained by the existence of promoter hypermethylation. To test this hypothesis, we integrated the epigenome and transcriptome data from a similar set of colorectal tissue samples. Methylation profiling was performed using the Illumina InfiniumHumanMethylation27 BeadChip on 55 paired cancer and adjacent normal epithelial cells. Fifteen of the 55 paired tissues were used for gene expression profiling using the Affymetrix GeneChip Human Gene 1.0 ST array. Validation was carried out on 150 colorectal tissues using the methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) technique. PCA and supervised hierarchical clustering in the two microarray datasets showed good separation between cancer and normal samples. Significant genes from the two analyses were obtained based on a ≥2-fold change and a false discovery rate (FDR) p-value of <0.05. We identified 1,081 differentially hypermethylated CpG sites and 36 hypomethylated CpG sites. We also found 709 upregulated and 699 downregulated genes from the gene expression profiling. A comparison of the two datasets revealed 32 overlapping genes with 27 being hypermethylated with downregulated expression and 4 hypermethylated with upregulated expression. One gene was found to be hypomethylated and downregulated. The most enriched molecular pathway identified was cell adhesion molecules that involved 4 overlapped genes, JAM2, NCAM1, ITGA8 and CNTN1. In the present study, we successfully identified a group of genes that showed methylation and gene expression changes in well-defined colorectal cancer tissues with high purity. The integrated analysis gives additional insight regarding the regulation of colorectal cancer-associated genes and their underlying mechanisms that contribute to colorectal carcinogenesis." @default.
• W1513314547 created "2016-06-24" @default.
• W1513314547 creator A5014900424 @default.
• W1513314547 creator A5017418421 @default.
• W1513314547 creator A5024799414 @default.
• W1513314547 creator A5029577017 @default.
• W1513314547 creator A5056702909 @default.
• W1513314547 creator A5058779000 @default.
• W1513314547 creator A5067240224 @default.
• W1513314547 date "2015-05-19" @default.
• W1513314547 modified "2023-09-23" @default.
• W1513314547 title "Identification of diagnostic markers in colorectal cancer via integrative epigenomics and genomics data" @default.
• W1513314547 cites W1965262464 @default.
• W1513314547 cites W1967826363 @default.
• W1513314547 cites W1982217924 @default.
• W1513314547 cites W1994322947 @default.
• W1513314547 cites W1994473465 @default.
• W1513314547 cites W1994629807 @default.
• W1513314547 cites W2007144721 @default.
• W1513314547 cites W2014764685 @default.
• W1513314547 cites W2023461603 @default.
• W1513314547 cites W2027060710 @default.
• W1513314547 cites W2029575827 @default.
• W1513314547 cites W2031842191 @default.
• W1513314547 cites W2041686632 @default.
• W1513314547 cites W2053758959 @default.
• W1513314547 cites W2056975833 @default.
• W1513314547 cites W2063608923 @default.
• W1513314547 cites W2064084554 @default.
• W1513314547 cites W2071328133 @default.
• W1513314547 cites W2072421397 @default.
• W1513314547 cites W2074124878 @default.
• W1513314547 cites W2083012142 @default.
• W1513314547 cites W2083020847 @default.
• W1513314547 cites W2101170994 @default.
• W1513314547 cites W2103603690 @default.
• W1513314547 cites W2105703073 @default.
• W1513314547 cites W2106441499 @default.
• W1513314547 cites W2107332277 @default.
• W1513314547 cites W2107396952 @default.
• W1513314547 cites W2107821075 @default.
• W1513314547 cites W2109100311 @default.
• W1513314547 cites W2109453679 @default.
• W1513314547 cites W2115444345 @default.
• W1513314547 cites W2115578647 @default.
• W1513314547 cites W2117996813 @default.
• W1513314547 cites W2119841500 @default.
• W1513314547 cites W2121417064 @default.
• W1513314547 cites W2123174009 @default.
• W1513314547 cites W2129655015 @default.
• W1513314547 cites W2131474316 @default.
• W1513314547 cites W2134164661 @default.
• W1513314547 cites W2137505881 @default.
• W1513314547 cites W2140380185 @default.
• W1513314547 cites W2141410747 @default.
• W1513314547 cites W2144137532 @default.
• W1513314547 cites W2144692208 @default.
• W1513314547 cites W2146304097 @default.
• W1513314547 cites W2149303776 @default.
• W1513314547 cites W2152316967 @default.
• W1513314547 cites W2152567646 @default.
• W1513314547 cites W2154336684 @default.
• W1513314547 cites W2156392314 @default.
• W1513314547 cites W2158021558 @default.
• W1513314547 cites W2158969533 @default.
• W1513314547 cites W2160704282 @default.
• W1513314547 cites W2161252246 @default.
• W1513314547 cites W2161429908 @default.
• W1513314547 cites W2161724145 @default.
• W1513314547 cites W2164847390 @default.
• W1513314547 cites W2257868630 @default.
• W1513314547 cites W2262414037 @default.
• W1513314547 cites W2337673235 @default.
• W1513314547 cites W4213257593 @default.
• W1513314547 cites W83864767 @default.
• W1513314547 cites W89989044 @default.
• W1513314547 doi "https://doi.org/10.3892/or.2015.3993" @default.
• W1513314547 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4484611" @default.
• W1513314547 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25997610" @default.
• W1513314547 hasPublicationYear "2015" @default.
• W1513314547 type Work @default.
• W1513314547 sameAs 1513314547 @default.
• W1513314547 citedByCount "37" @default.
• W1513314547 countsByYear W15133145472015 @default.
• W1513314547 countsByYear W15133145472016 @default.
• W1513314547 countsByYear W15133145472017 @default.
• W1513314547 countsByYear W15133145472018 @default.
• W1513314547 countsByYear W15133145472019 @default.
• W1513314547 countsByYear W15133145472020 @default.
• W1513314547 countsByYear W15133145472021 @default.
• W1513314547 countsByYear W15133145472022 @default.
• W1513314547 countsByYear W15133145472023 @default.
• W1513314547 crossrefType "journal-article" @default.
• W1513314547 hasAuthorship W1513314547A5014900424 @default.
• W1513314547 hasAuthorship W1513314547A5017418421 @default.
• W1513314547 hasAuthorship W1513314547A5024799414 @default.
• W1513314547 hasAuthorship W1513314547A5029577017 @default.
• W1513314547 hasAuthorship W1513314547A5056702909 @default.

• next