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- W1513444711 abstract "A new solid-state model of nicked DNA was used as a substrate for polynucleotide ligases from various sources. It consisted of a complementary poly(dA) · poly(dT) duplex in which the light strand was split by a 4′-phosphate//3′-hydroxyl interruption into two portions: one was labeled with 3H and the other was nonradioactive and was grafted onto cellulose particles. The enzyme sealed the breaks thus linking covalently the poly([3H]dT) chains to the cellulose which could then be isolated from the unreacted material by centrifugation in alkali and counted for radioactivity. As described in detail, the system was prepared by simple biochemical procedures and with the aid of a single enzyme, the terminal deoxynucleotidyl transferase from calf thymus." @default.
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- W1513444711 date "1971-07-01" @default.
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- W1513444711 title "A convenient solid-state substrate for assays of polynucleotide ligase" @default.
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- W1513444711 doi "https://doi.org/10.1016/0005-2787(71)90708-8" @default.
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