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- W151367923 abstract "A synthetic peptide of 21 amino acids, homologous to the hinge of the human IgG2 molecule was used for immunization of mice to produce anti-IgG-2 subclass specific monoclonal antibodies. Two monoclonal antibodies of mouse IgG1 and IgM isotype were produced recognizing the IgG2 molecule under various assay conditions. The determinants recognized are located on the Fc and Fab portion of the heavy chain respectively. The IgG2 specific amino acid substitutions are located on the carboxy terminal part of the peptide. Immunization with this determinant, in a short peptide (10 amino acids), on itself did not lead to IgG2 specific antibody responses. A second determinant to the amino terminal side of the peptide to which specific antibodies could be produced appears necessary for the induction of structural elements mimicking the native protein" @default.
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- W151367923 date "1989-01-01" @default.
- W151367923 modified "2023-09-26" @default.
- W151367923 title "Anti-Peptide Antibodies as Subclass Specific Reagents: Epitope Mapping of Human IgG-2" @default.
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- W151367923 doi "https://doi.org/10.1016/b978-0-08-037378-2.50027-9" @default.
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