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- W1513684289 abstract "Bivalves are constantly exposed to different kinds of stressors as they live in a habitat with frequent changes in environmentalparameters. The xenobiotic pollutants also contribute to the stressful routine of bivalves. Studies on the genes which mediateand contribute to the physiological plasticity of bivalves in stressful situations, induced by natural and anthropogenic agentsare gaining importance. Among the stress related genes, HSP family genes play an important role in managing stress inducedby various factors. Recent reports underline the role of heat shock proteins in thermo tolerance, host defense and even inaging. Here we report the molecular expression and detection of heat shock protein genes (Hsp70) from the Indian edibleoyster Crassostrea madrasensis and the Indian brown mussel Perna indica with unique distribution in Indian waters. The c-DNA reverse transcribed from the total RNA of gill was used as template in Polymerase Chain Reaction (PCR) to amplifyHsp70 gene segments with primers designed from the conserved nucleotide sequences of Crassostrea gigas and Pernaviridis. PCR products were sequenced, and the similarity search in NCBI-BLAST confirmed the molecular identity oftargeted genes. Phylogenetic analysis of the Hsp gene sequence data reveals the unique position of the Indian edible oysterand Indian brown mussel among the other counterparts inhabiting rest of the world. This stands out as the first report on theexpression and PCR amplification of stress related genes from Indian bivalves." @default.
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- W1513684289 date "2012-12-28" @default.
- W1513684289 modified "2023-09-23" @default.
- W1513684289 title "Molecular identification of heat shock protein 70 (Hsp70) gene in the Indian edible oyster Crassostrea madrasensis (Preston) and Indian brown mussel Perna indica Kuriakose & Nair, 1976" @default.
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