FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1513971953> ?p ?o ?g. }

• W1513971953 endingPage "169" @default.
• W1513971953 startingPage "157" @default.
• W1513971953 abstract "Expression of the bcl-2 gene becomes deregulated in many non-Hodgkin lymphomas as the result of t(14;18) chromosomal translocations. Because bcl-2 regulates the survival of cells, and because its over-expression is associated with cellular resistance to killing by chemotherapeutic drugs and gamma-irradiation, this gene and its mRNA and protein products represent ideal targets for designing novel therapeutic strategies for the treatment of cancer. Here we describe the effects of an 18-mer phosphodiester oligonucleotide that is complementary to the first 6 codons of the bcl-2 mRNA's open reading frame. When tested for inhibition of in vitro protein synthesis using RNAse-H-supplemented reticulocyte lysates and RNA prepared by in vitro transcription of a human bcl-2 cDNA, the bcl-2 antisense (AS) oligomer completely abolished Bcl-2 protein production at 10 microM, but had no effect on the in vitro translation of a chicken bcl-2 RNA that contained three mismatches relative to the oligomer binding site on the human bcl-2 RNA. A control 18-mer having the same base composition as the AS oligomer but with scrambled order (SC) was not inhibitory. Addition of AS and SC oligomers to cultures of a NIH-3T3 fibroblast cell line that had been stably infected with a recombinant retrovirus containing the same human bcl-2 cDNA used for in vitro transcription/translation experiments revealed concentration-dependent reductions in the relative levels of the 26-kD human Bcl-2 protein (as determined by immunoblotting) by the AS but not by the SC oligomer. Similar results were obtained when AS and SC oligomers were applied to a t(14;18)-containing lymphoma cell line SU-DHL-4 that was cultured in low-serum media. When used at 200 microM, the bcl-2 AS oligomer produced 84-95% reductions in Bcl-2 protein levels in SU-DHL-4 cells but had relatively little effect on the levels of other mitochondrial control proteins, suggesting that the inhibitory effects were specific. Treatment of SU-DHL-4 cells with AS oligomer lead to essentially complete loss of bcl-2 mRNA from cells within 1 day of addition to cultures, but presumably because of the long half-life of the Bcl-2 protein (approximately 14 h), commensurate reductions in Bcl-2 protein levels did not occur until 3 days.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W1513971953 created "2016-06-24" @default.
• W1513971953 creator A5033689092 @default.
• W1513971953 creator A5035082589 @default.
• W1513971953 creator A5059438505 @default.
• W1513971953 creator A5060485140 @default.
• W1513971953 date "1993-01-01" @default.
• W1513971953 modified "2023-10-14" @default.
• W1513971953 title "Investigations of Antisense Oligonucleotides Targeted Against<i>bcl-2</i>RNAs" @default.
• W1513971953 cites W1513433026 @default.
• W1513971953 cites W1568678893 @default.
• W1513971953 cites W1589415602 @default.
• W1513971953 cites W1591166893 @default.
• W1513971953 cites W1971234510 @default.
• W1513971953 cites W1981663445 @default.
• W1513971953 cites W2003501749 @default.
• W1513971953 cites W2004631966 @default.
• W1513971953 cites W2008898220 @default.
• W1513971953 cites W2009183764 @default.
• W1513971953 cites W2015856337 @default.
• W1513971953 cites W2033214263 @default.
• W1513971953 cites W2043066069 @default.
• W1513971953 cites W2048428832 @default.
• W1513971953 cites W2057257609 @default.
• W1513971953 cites W2063915751 @default.
• W1513971953 cites W2066566138 @default.
• W1513971953 cites W2075576299 @default.
• W1513971953 cites W2078119356 @default.
• W1513971953 cites W2078457332 @default.
• W1513971953 cites W2084565769 @default.
• W1513971953 cites W2134812217 @default.
• W1513971953 cites W2139201710 @default.
• W1513971953 cites W2144808732 @default.
• W1513971953 cites W2156276815 @default.
• W1513971953 cites W4229992754 @default.
• W1513971953 cites W4246653283 @default.
• W1513971953 doi "https://doi.org/10.1089/ard.1993.3.157" @default.
• W1513971953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8400801" @default.
• W1513971953 hasPublicationYear "1993" @default.
• W1513971953 type Work @default.
• W1513971953 sameAs 1513971953 @default.
• W1513971953 citedByCount "93" @default.
• W1513971953 countsByYear W15139719532012 @default.
• W1513971953 countsByYear W15139719532013 @default.
• W1513971953 countsByYear W15139719532014 @default.
• W1513971953 countsByYear W15139719532015 @default.
• W1513971953 countsByYear W15139719532016 @default.
• W1513971953 countsByYear W15139719532018 @default.
• W1513971953 countsByYear W15139719532022 @default.
• W1513971953 crossrefType "journal-article" @default.
• W1513971953 hasAuthorship W1513971953A5033689092 @default.
• W1513971953 hasAuthorship W1513971953A5035082589 @default.
• W1513971953 hasAuthorship W1513971953A5059438505 @default.
• W1513971953 hasAuthorship W1513971953A5060485140 @default.
• W1513971953 hasConcept C104317684 @default.
• W1513971953 hasConcept C105580179 @default.
• W1513971953 hasConcept C10879258 @default.
• W1513971953 hasConcept C129312508 @default.
• W1513971953 hasConcept C138885662 @default.
• W1513971953 hasConcept C150194340 @default.
• W1513971953 hasConcept C153911025 @default.
• W1513971953 hasConcept C179926584 @default.
• W1513971953 hasConcept C187882448 @default.
• W1513971953 hasConcept C202751555 @default.
• W1513971953 hasConcept C3675279 @default.
• W1513971953 hasConcept C41895202 @default.
• W1513971953 hasConcept C55493867 @default.
• W1513971953 hasConcept C67705224 @default.
• W1513971953 hasConcept C69766542 @default.
• W1513971953 hasConcept C86803240 @default.
• W1513971953 hasConceptScore W1513971953C104317684 @default.
• W1513971953 hasConceptScore W1513971953C105580179 @default.
• W1513971953 hasConceptScore W1513971953C10879258 @default.
• W1513971953 hasConceptScore W1513971953C129312508 @default.
• W1513971953 hasConceptScore W1513971953C138885662 @default.
• W1513971953 hasConceptScore W1513971953C150194340 @default.
• W1513971953 hasConceptScore W1513971953C153911025 @default.
• W1513971953 hasConceptScore W1513971953C179926584 @default.
• W1513971953 hasConceptScore W1513971953C187882448 @default.
• W1513971953 hasConceptScore W1513971953C202751555 @default.
• W1513971953 hasConceptScore W1513971953C3675279 @default.
• W1513971953 hasConceptScore W1513971953C41895202 @default.
• W1513971953 hasConceptScore W1513971953C55493867 @default.
• W1513971953 hasConceptScore W1513971953C67705224 @default.
• W1513971953 hasConceptScore W1513971953C69766542 @default.
• W1513971953 hasConceptScore W1513971953C86803240 @default.
• W1513971953 hasIssue "2" @default.
• W1513971953 hasLocation W15139719531 @default.
• W1513971953 hasLocation W15139719532 @default.
• W1513971953 hasOpenAccess W1513971953 @default.
• W1513971953 hasPrimaryLocation W15139719531 @default.
• W1513971953 hasRelatedWork W1626305077 @default.
• W1513971953 hasRelatedWork W1988693697 @default.
• W1513971953 hasRelatedWork W2002529280 @default.
• W1513971953 hasRelatedWork W2016147415 @default.
• W1513971953 hasRelatedWork W2038365032 @default.
• W1513971953 hasRelatedWork W2080304317 @default.
• W1513971953 hasRelatedWork W2092554673 @default.

• next