FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1515344094> ?p ?o ?g. }

• W1515344094 endingPage "100" @default.
• W1515344094 startingPage "95" @default.
• W1515344094 abstract "Hrq1은 곰팡이 유전체에서 생물정보분석에 의해 발견된 새로운 RecQ helicase이다. 이 단백질은 인간의 RECQL4와 가장 상동성이 높으며 최근의 유전학적 생화학적 연구를 통해서 유전체 안정성을 유지하는데 어떤 역할을 할 것으로 예상되었다. 본 연구에서는 RECQL4와 상호작용하는 것으로 알려진 인간 유전자들과 상동성이 있는 효모 유전자들이 Hrq1과 상호작용하는지를 yeast two-hybrid assay를 이용하여 조사하였다. 총 11개의 유전자를 조사한 결과, nucleotide excision repair (NER) 인자 중의 하나인 Rad14이 Hrq1과 상호작용하는 것을 발견하였다. 또한 정제한 단백질을 이용한 pull-down assay로 Hrq1과 Rad14 사이의 직접적인 상호작용을 확인하였다. Hrq1과 Rad14 사이의 yeast two-hybrid 상호작용은 4-nitroquinoline-1-oxide에 의한 DNA 손상으로 더욱 증가하였으며, 이러한 상호작용의 증가는 또 다른 NER 인자인 Rad4에 의존적이었다. 이러한 결과들은 Hrq1이 Rad14과의 상호작용을 통하여 NER 과정에 어떤 역할을 할 가능성을 제시하고 있다. Hrq1 is a novel member of RecQ helicase family, found in fungal genomes by bioinformatics analyses. It is most homologous to human RECQL4 and recent genetic and biochemical studies suggested that it may play roles in the maintenance of genome stability. In this study, we investigated yeast two-hybrid interactions between Hrq1 and the yeast genes homologous to the human genes that are known to interact with RECQL4. Among the 11 genes tested, Rad14, a nucleotide excision repair (NER) factor, was found to interact with Hrq1. In addition, pull-down assay with the purified proteins revealed direct protein-protein interaction between Hrq1 and Rad14. The yeast two-hybrid interaction was enhanced by the DNA damage induced by 4-nitroquinoline-1-oxide, which was dependent on the presence of Rad4, a key NER factor. These results suggest that Hrq1 may function in NER through interaction with Rad14." @default.
• W1515344094 created "2016-06-24" @default.
• W1515344094 creator A5005949828 @default.
• W1515344094 creator A5029843639 @default.
• W1515344094 creator A5049839002 @default.
• W1515344094 creator A5063818797 @default.
• W1515344094 creator A5067482044 @default.
• W1515344094 creator A5071769432 @default.
• W1515344094 creator A5081522602 @default.
• W1515344094 creator A5086257602 @default.
• W1515344094 creator A5073734139 @default.
• W1515344094 date "2014-06-30" @default.
• W1515344094 modified "2023-10-17" @default.
• W1515344094 title "Characterization of Hrq1-Rad14 Interaction in Saccharomyces cerevisiae" @default.
• W1515344094 cites W1526043578 @default.
• W1515344094 cites W1819499229 @default.
• W1515344094 cites W1903792504 @default.
• W1515344094 cites W1967190671 @default.
• W1515344094 cites W1968676497 @default.
• W1515344094 cites W2002900988 @default.
• W1515344094 cites W2006338892 @default.
• W1515344094 cites W2029995604 @default.
• W1515344094 cites W2039876512 @default.
• W1515344094 cites W2041514249 @default.
• W1515344094 cites W2043505940 @default.
• W1515344094 cites W2045464664 @default.
• W1515344094 cites W2055130983 @default.
• W1515344094 cites W2062113330 @default.
• W1515344094 cites W2064449916 @default.
• W1515344094 cites W2067286200 @default.
• W1515344094 cites W2070067291 @default.
• W1515344094 cites W2077038149 @default.
• W1515344094 cites W2080408951 @default.
• W1515344094 cites W2080726979 @default.
• W1515344094 cites W2082918267 @default.
• W1515344094 cites W2127392515 @default.
• W1515344094 cites W2143472729 @default.
• W1515344094 cites W2156354811 @default.
• W1515344094 cites W2161776138 @default.
• W1515344094 doi "https://doi.org/10.7845/kjm.2014.4016" @default.
• W1515344094 hasPublicationYear "2014" @default.
• W1515344094 type Work @default.
• W1515344094 sameAs 1515344094 @default.
• W1515344094 citedByCount "0" @default.
• W1515344094 crossrefType "journal-article" @default.
• W1515344094 hasAuthorship W1515344094A5005949828 @default.
• W1515344094 hasAuthorship W1515344094A5029843639 @default.
• W1515344094 hasAuthorship W1515344094A5049839002 @default.
• W1515344094 hasAuthorship W1515344094A5063818797 @default.
• W1515344094 hasAuthorship W1515344094A5067482044 @default.
• W1515344094 hasAuthorship W1515344094A5071769432 @default.
• W1515344094 hasAuthorship W1515344094A5073734139 @default.
• W1515344094 hasAuthorship W1515344094A5081522602 @default.
• W1515344094 hasAuthorship W1515344094A5086257602 @default.
• W1515344094 hasBestOaLocation W15153440941 @default.
• W1515344094 hasConcept C102744134 @default.
• W1515344094 hasConcept C104317684 @default.
• W1515344094 hasConcept C104451858 @default.
• W1515344094 hasConcept C134935766 @default.
• W1515344094 hasConcept C161223559 @default.
• W1515344094 hasConcept C2777576037 @default.
• W1515344094 hasConcept C2779222958 @default.
• W1515344094 hasConcept C2780751114 @default.
• W1515344094 hasConcept C54355233 @default.
• W1515344094 hasConcept C552990157 @default.
• W1515344094 hasConcept C64894306 @default.
• W1515344094 hasConcept C67705224 @default.
• W1515344094 hasConcept C68592252 @default.
• W1515344094 hasConcept C86803240 @default.
• W1515344094 hasConceptScore W1515344094C102744134 @default.
• W1515344094 hasConceptScore W1515344094C104317684 @default.
• W1515344094 hasConceptScore W1515344094C104451858 @default.
• W1515344094 hasConceptScore W1515344094C134935766 @default.
• W1515344094 hasConceptScore W1515344094C161223559 @default.
• W1515344094 hasConceptScore W1515344094C2777576037 @default.
• W1515344094 hasConceptScore W1515344094C2779222958 @default.
• W1515344094 hasConceptScore W1515344094C2780751114 @default.
• W1515344094 hasConceptScore W1515344094C54355233 @default.
• W1515344094 hasConceptScore W1515344094C552990157 @default.
• W1515344094 hasConceptScore W1515344094C64894306 @default.
• W1515344094 hasConceptScore W1515344094C67705224 @default.
• W1515344094 hasConceptScore W1515344094C68592252 @default.
• W1515344094 hasConceptScore W1515344094C86803240 @default.
• W1515344094 hasIssue "2" @default.
• W1515344094 hasLocation W15153440941 @default.
• W1515344094 hasOpenAccess W1515344094 @default.
• W1515344094 hasPrimaryLocation W15153440941 @default.
• W1515344094 hasRelatedWork W1515344094 @default.
• W1515344094 hasRelatedWork W1996161238 @default.
• W1515344094 hasRelatedWork W2051544241 @default.
• W1515344094 hasRelatedWork W2056445557 @default.
• W1515344094 hasRelatedWork W2070067291 @default.
• W1515344094 hasRelatedWork W2085975659 @default.
• W1515344094 hasRelatedWork W2093138085 @default.
• W1515344094 hasRelatedWork W2417422633 @default.
• W1515344094 hasRelatedWork W256913593 @default.
• W1515344094 hasRelatedWork W4246228642 @default.
• W1515344094 hasVolume "50" @default.

• next