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• W1515664586 abstract "The aim of the present work was to determine and compare the degradation of acetate in a Chinese rice field soil at 25°C and 50°C, respectively, and to identify specifically the active organisms involved in syntrophic acetate oxidation. Soil was preincubated anaerobically for 30 days to reduce alternative electron acceptors other than CO(2). The [2-(13)C] acetate (99% (13)C) was added twice: 0 day and 19 days after preincubation. Addition of [2-(13)C] acetate resulted in an immediate increase of (13)C labeled CH(4) but non-labeling of CO(2) at 25°C. The methanogen community was dominated by Methanosarcinaceae and Methanocellales at 25°C. In contrast, the addition of [2-(13)C] acetate at 50°C resulted in a rapid increase of (13)CO(2). The (13)C labeling of CH(4) gradually increased and reached a similar value to CO(2) (13% (13)C) at the end of incubation (40 days). Nearly all archaeal 16S rRNA genes detected at 50°C belonged to hydrogenotrophic Methanocellales. DNA-based stable isotope probing analysis revealed that the organisms related to Thermacetogenium lineage and the unclassified Thermoanaerobacteraceae group were intensively labeled with (13)C in the incubations at 50°C. Thus, acetate was converted to CH(4) and CO(2) through aceticlastic methanogenesis at 25°C, while syntrophic acetate oxidation occurred at 50°C." @default.
• W1515664586 created "2016-06-24" @default.
• W1515664586 creator A5015024432 @default.
• W1515664586 creator A5030671298 @default.
• W1515664586 creator A5044227390 @default.
• W1515664586 date "2011-05-05" @default.
• W1515664586 modified "2023-10-12" @default.
• W1515664586 title "Syntrophic acetate oxidation under thermophilic methanogenic condition in Chinese paddy field soil" @default.
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• W1515664586 doi "https://doi.org/10.1111/j.1574-6941.2011.01104.x" @default.
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