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- W1515671137 abstract "Developing sand dollar eggs were treated with actinomycin D, 5-bromodeoxyuridine (BUdR), chloramphenicol or mercaptoethanol for carefully controlled periods of time, prior to second cleavage and assayed for mature centrioles by the indirect method involving benzimidazole. No electron microscope observations were used. All four of these reagents interfered with centriole duplication. However, the time pattern of interference by chloramphenicol or mercaptoethanol was distinctly and consistently different from that observed with actinomycin and bromodeoxyuridine. Thymidine in excess neutralized the presence of bromodeoxyuridine. The data support the “generative” mechanism for centriole duplication suggested by Mazia. An elaboration of this model is presented. It is also hypothesized that the primary genome for centrioles is in the form of RNA which is replicated via a DNA intermediate during the generative phase of the centriole duplication cycle." @default.
- W1515671137 created "2016-06-24" @default.
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- W1515671137 date "1977-08-01" @default.
- W1515671137 modified "2023-09-23" @default.
- W1515671137 title "Centriole duplication in sand dollar eggs" @default.
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- W1515671137 doi "https://doi.org/10.1016/s0014-4827(77)80010-4" @default.
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