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- W1515989763 abstract "Recovery of hydrophobic proteins from an RP-HPLC column was improved using a fast-separation RP-HPLC system operated at room temperature. Hydrophobic proteins such as ovalbumin could be adequately eluted from a nonporous octadecylsilyl (C18) spherical silica gel with a particle diameter of 20 μm using steep gradient elution with a 0.1% aqueous trifluoroacetic acid-acetonitrile system at a constant flow rate of 4 ml/min. Recoveries improved under fast separation since the protein sample suffered only a slight amount of irreversible denaturation on the hydrophobic surface of the stationary phase. The fast-separation system was also applied to the separation of larger proteins such as apoferritin (443 kDa) and thyroglobulin (669 kDa) as well as egg white proteins." @default.
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- W1515989763 date "1991-11-01" @default.
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- W1515989763 title "Fast protein separation by reversed-phase high-performance liquid chromatography on octadecylsilyl-bonded nonporous silica gel" @default.
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- W1515989763 doi "https://doi.org/10.1016/0003-2697(91)90261-q" @default.
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