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• W1516564694 abstract "Abstract The purified serogroup X meningococcal polysaccharide contains equimolar proportions of 2-acetamido-2-deoxy-d- glucose and phosphate as its sole constituents. Under conditions of mild acid hydrolysis the polysaccharide yielded only 2-acetamido-2-deoxy-d-glucose 4-phosphate, indicating that the adjacent acetamido sugar units are linked by 1→4 phosphodiester bonds. Other studies on the polysaccharide indicate that it is linear, has an average chain length of 50 units, and that the glycosidic phosphodiester bonds are in the α configuration. Serological studies on the polysaccharide have shown that its antiserum is highly specific for the position of linkage of the phosphodiester bonds. Also, the poor inhibitory properties, to the precipitin reaction, of the α-1-phosphate and 4-phosphate monomers indicate that the main serological specificity is probably directed to oligomer units. The polysaccharide was isolated by a method involving buffered phenol extraction, to remove protein contaminants, with subsequent treatment by ribonuclease, to remove nucleic acid. This rapid and simple procedure is adaptable to the isolation of other meningococcal polysaccharides (serogroups A, B, C, Y, and Z) in both good yields and a high degree of purity." @default.
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• W1516564694 date "1974-08-01" @default.
• W1516564694 modified "2023-10-18" @default.
• W1516564694 title "Studies on the Group-specific Polysaccharide of Neisseria meningitidis Serogroup X and an Improved Procedure for Its Isolation" @default.
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• W1516564694 doi "https://doi.org/10.1016/s0021-9258(19)42390-9" @default.
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