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• W1516996883 abstract "Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:681–687, 2013" @default.
• W1516996883 created "2016-06-24" @default.
• W1516996883 creator A5053676002 @default.
• W1516996883 creator A5082774427 @default.
• W1516996883 date "2013-04-18" @default.
• W1516996883 modified "2023-10-02" @default.
• W1516996883 title "Separation, antitumor activities, and encapsulation of polypeptide from<i>Chlorella pyrenoidosa</i>" @default.
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• W1516996883 doi "https://doi.org/10.1002/btpr.1725" @default.
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