Matches in SemOpenAlex for { <https://semopenalex.org/work/W1517283541> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1517283541 endingPage "15618" @default.
- W1517283541 startingPage "15615" @default.
- W1517283541 abstract "For the first time, the enzyme rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) has been renatured from 6 M guanidinium chloride (GdmCl) by direct dilution of the denaturant at relatively high protein concentrations. This has been made possible by using the nonionic detergent dodecyl-beta-D-maltoside (lauryl maltoside). Lauryl maltoside concentration dependence of the renaturation and reactivation time courses were studied using 50 micrograms/ml rhodanese. There was no renaturation at lauryl maltoside (less than 0.1 mg/ml), and the renaturability increased, apparently cooperatively, up to 5 mg/ml detergent. This may reflect weak binding of lauryl maltoside to intermediate rhodanese conformers. The renaturability began to decrease above 5 mg/ml lauryl maltoside and was significantly reduced at 20 mg/ml. Individual progress curves of product formation, for rhodanese diluted into lauryl maltoside 90 min before assay, showed induction phases as long as 7 min before an apparently linear steady state. The induction phase increased with lauryl maltoside concentration and could even be observed in native controls above 1 mg/ml detergent. These results are consistent with suggestions that refolding of GdmCl-denatured rhodanese involves an intermediate with exposed hydrophobic surfaces that can partition into active and inactive species. Further, lauryl maltoside can stabilize those surfaces and prevent aggregation and other hydrophobic interaction-dependent events that reduce the yield of active protein. The rhodanese-lauryl maltoside complex could also form with native enzyme, thus explaining the induction phase with this species. Finally, it is suggested that renaturation of many proteins might be assisted by lauryl maltoside or other nondenaturing detergents." @default.
- W1517283541 created "2016-06-24" @default.
- W1517283541 creator A5035578813 @default.
- W1517283541 creator A5082418225 @default.
- W1517283541 date "1986-11-01" @default.
- W1517283541 modified "2023-10-18" @default.
- W1517283541 title "Detergent-assisted refolding of guanidinium chloride-denatured rhodanese. The effect of lauryl maltoside." @default.
- W1517283541 cites W1507400693 @default.
- W1517283541 cites W1520122363 @default.
- W1517283541 cites W1606605016 @default.
- W1517283541 cites W1966991787 @default.
- W1517283541 cites W1982936667 @default.
- W1517283541 cites W1987419412 @default.
- W1517283541 cites W1991461113 @default.
- W1517283541 cites W1994342401 @default.
- W1517283541 cites W2030296263 @default.
- W1517283541 cites W2046294105 @default.
- W1517283541 cites W2086731700 @default.
- W1517283541 cites W2125625153 @default.
- W1517283541 cites W2206318246 @default.
- W1517283541 doi "https://doi.org/10.1016/s0021-9258(18)66759-6" @default.
- W1517283541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3465721" @default.
- W1517283541 hasPublicationYear "1986" @default.
- W1517283541 type Work @default.
- W1517283541 sameAs 1517283541 @default.
- W1517283541 citedByCount "121" @default.
- W1517283541 countsByYear W15172835412012 @default.
- W1517283541 countsByYear W15172835412013 @default.
- W1517283541 countsByYear W15172835412015 @default.
- W1517283541 countsByYear W15172835412016 @default.
- W1517283541 countsByYear W15172835412017 @default.
- W1517283541 countsByYear W15172835412018 @default.
- W1517283541 countsByYear W15172835412020 @default.
- W1517283541 countsByYear W15172835412021 @default.
- W1517283541 countsByYear W15172835412023 @default.
- W1517283541 crossrefType "journal-article" @default.
- W1517283541 hasAuthorship W1517283541A5035578813 @default.
- W1517283541 hasAuthorship W1517283541A5082418225 @default.
- W1517283541 hasBestOaLocation W15172835411 @default.
- W1517283541 hasConcept C178790620 @default.
- W1517283541 hasConcept C181199279 @default.
- W1517283541 hasConcept C185592680 @default.
- W1517283541 hasConcept C2777654166 @default.
- W1517283541 hasConcept C2778695967 @default.
- W1517283541 hasConcept C2779033446 @default.
- W1517283541 hasConcept C43617362 @default.
- W1517283541 hasConcept C55493867 @default.
- W1517283541 hasConceptScore W1517283541C178790620 @default.
- W1517283541 hasConceptScore W1517283541C181199279 @default.
- W1517283541 hasConceptScore W1517283541C185592680 @default.
- W1517283541 hasConceptScore W1517283541C2777654166 @default.
- W1517283541 hasConceptScore W1517283541C2778695967 @default.
- W1517283541 hasConceptScore W1517283541C2779033446 @default.
- W1517283541 hasConceptScore W1517283541C43617362 @default.
- W1517283541 hasConceptScore W1517283541C55493867 @default.
- W1517283541 hasIssue "33" @default.
- W1517283541 hasLocation W15172835411 @default.
- W1517283541 hasOpenAccess W1517283541 @default.
- W1517283541 hasPrimaryLocation W15172835411 @default.
- W1517283541 hasRelatedWork W1517283541 @default.
- W1517283541 hasRelatedWork W1517296610 @default.
- W1517283541 hasRelatedWork W1520122363 @default.
- W1517283541 hasRelatedWork W1606605016 @default.
- W1517283541 hasRelatedWork W1990559022 @default.
- W1517283541 hasRelatedWork W1996591246 @default.
- W1517283541 hasRelatedWork W2084959393 @default.
- W1517283541 hasRelatedWork W2415654683 @default.
- W1517283541 hasRelatedWork W2605909717 @default.
- W1517283541 hasRelatedWork W2327432447 @default.
- W1517283541 hasVolume "261" @default.
- W1517283541 isParatext "false" @default.
- W1517283541 isRetracted "false" @default.
- W1517283541 magId "1517283541" @default.
- W1517283541 workType "article" @default.