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- W1517507751 abstract "Present study reports the use of randomly amplified polymorphic DNA (RAPD) markers, to verify the clonal fidelity between the micropropagated plants and the mother plant of Ochreinauclea missionis, an ethnomedicinal and endemic tree in Western Ghats of India. Thirty eight RAPD primers were screened, out of which thirty two primers generated a total of 245 clear, distinct and reproducible bands. Out of 245 total bands, 227 bands were monomorphic with 92.67% and 18 bands showed polymorphic banding pattern with 7.34%. Thus, a total of 5390 bands were generated showing uniform banding patterns for each primer that are comparable to the mother plant from which the cultures were established. Cluster analysis based on unweighted pair group method with arithmetic averaging (UPGMA) showed 97% similarity between the mother plant and micropropagated plants. The developed RAPD profiles confirmed the clonal fidelity of tissue culture raised plantlets which were reintroduced to its original habitat for conservation." @default.
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- W1517507751 date "2009-01-01" @default.
- W1517507751 modified "2023-09-27" @default.
- W1517507751 title "An assessment of genetic stability in micropropagated plants of Ochreinauclea missionis by RAPD markers" @default.
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