Matches in SemOpenAlex for { <https://semopenalex.org/work/W1517822256> ?p ?o ?g. }
- W1517822256 endingPage "2341" @default.
- W1517822256 startingPage "2332" @default.
- W1517822256 abstract "Starting with oligopeptide sequences and using PCR, the gene of the cyclodextrinase from Flavobacterium sp. no. 92 was derived from the genomic DNA. The gene was sequenced and expressed in Escherichia coli ; the gene product was purified and crystallized. An X‐ray diffraction analysis using seleno‐methionines with multiwavelength anomalous diffraction techniques yielded the refined 3D structure at 2.1 Å resolution. The enzyme hydrolyzes α(1,4)‐glycosidic bonds of cyclodextrins and linear malto‐oligosaccharides. It belongs to the glycosylhydrolase family no. 13 and has a chain fold similar to that of α‐amylases, cyclodextrin glycosyltransferases, and other cyclodextrinases. In contrast with most family members but in agreement with other cyclodextrinases, the enzyme contains an additional characteristic N‐terminal domain of about 100 residues. This domain participates in the formation of a putative D 2 ‐symmetric tetramer but not in cyclodextrin binding at the active center as observed with the other cyclodextrinases. Moreover, the domain is located at a position quite different from that of the other cyclodextrinases. Whether oligomerization facilitates the cyclodextrin deformation required for hydrolysis is discussed." @default.
- W1517822256 created "2016-06-24" @default.
- W1517822256 creator A5025542887 @default.
- W1517822256 creator A5049569891 @default.
- W1517822256 creator A5071727085 @default.
- W1517822256 date "2003-04-25" @default.
- W1517822256 modified "2023-10-17" @default.
- W1517822256 title "Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92" @default.
- W1517822256 cites W1506811553 @default.
- W1517822256 cites W1539796472 @default.
- W1517822256 cites W1575814752 @default.
- W1517822256 cites W1591680814 @default.
- W1517822256 cites W1830745998 @default.
- W1517822256 cites W1918349277 @default.
- W1517822256 cites W1969153543 @default.
- W1517822256 cites W1971258699 @default.
- W1517822256 cites W1977709772 @default.
- W1517822256 cites W1983389428 @default.
- W1517822256 cites W1988952661 @default.
- W1517822256 cites W1991882432 @default.
- W1517822256 cites W1992705386 @default.
- W1517822256 cites W1995017064 @default.
- W1517822256 cites W1996007886 @default.
- W1517822256 cites W1998869143 @default.
- W1517822256 cites W1999957822 @default.
- W1517822256 cites W2001358446 @default.
- W1517822256 cites W2001641653 @default.
- W1517822256 cites W2004904454 @default.
- W1517822256 cites W2007276478 @default.
- W1517822256 cites W2013083986 @default.
- W1517822256 cites W2014669590 @default.
- W1517822256 cites W2016451409 @default.
- W1517822256 cites W2016549023 @default.
- W1517822256 cites W2028231353 @default.
- W1517822256 cites W2046017608 @default.
- W1517822256 cites W2046319395 @default.
- W1517822256 cites W2049302282 @default.
- W1517822256 cites W2054624750 @default.
- W1517822256 cites W2056653261 @default.
- W1517822256 cites W2080528351 @default.
- W1517822256 cites W2084459566 @default.
- W1517822256 cites W2086056666 @default.
- W1517822256 cites W2092987196 @default.
- W1517822256 cites W2095237995 @default.
- W1517822256 cites W2101749711 @default.
- W1517822256 cites W2110958200 @default.
- W1517822256 cites W2112200653 @default.
- W1517822256 cites W2117538889 @default.
- W1517822256 cites W2119546811 @default.
- W1517822256 cites W2133552794 @default.
- W1517822256 cites W2135464698 @default.
- W1517822256 cites W2140720902 @default.
- W1517822256 cites W2409474219 @default.
- W1517822256 doi "https://doi.org/10.1046/j.1432-1033.2003.03603.x" @default.
- W1517822256 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12752453" @default.
- W1517822256 hasPublicationYear "2003" @default.
- W1517822256 type Work @default.
- W1517822256 sameAs 1517822256 @default.
- W1517822256 citedByCount "35" @default.
- W1517822256 countsByYear W15178222562012 @default.
- W1517822256 countsByYear W15178222562013 @default.
- W1517822256 countsByYear W15178222562014 @default.
- W1517822256 countsByYear W15178222562015 @default.
- W1517822256 countsByYear W15178222562016 @default.
- W1517822256 countsByYear W15178222562017 @default.
- W1517822256 countsByYear W15178222562018 @default.
- W1517822256 countsByYear W15178222562019 @default.
- W1517822256 countsByYear W15178222562021 @default.
- W1517822256 countsByYear W15178222562022 @default.
- W1517822256 countsByYear W15178222562023 @default.
- W1517822256 crossrefType "journal-article" @default.
- W1517822256 hasAuthorship W1517822256A5025542887 @default.
- W1517822256 hasAuthorship W1517822256A5049569891 @default.
- W1517822256 hasAuthorship W1517822256A5071727085 @default.
- W1517822256 hasBestOaLocation W15178222561 @default.
- W1517822256 hasConcept C104317684 @default.
- W1517822256 hasConcept C150487720 @default.
- W1517822256 hasConcept C161019410 @default.
- W1517822256 hasConcept C181199279 @default.
- W1517822256 hasConcept C185592680 @default.
- W1517822256 hasConcept C2778886173 @default.
- W1517822256 hasConcept C547475151 @default.
- W1517822256 hasConcept C55493867 @default.
- W1517822256 hasConcept C71240020 @default.
- W1517822256 hasConceptScore W1517822256C104317684 @default.
- W1517822256 hasConceptScore W1517822256C150487720 @default.
- W1517822256 hasConceptScore W1517822256C161019410 @default.
- W1517822256 hasConceptScore W1517822256C181199279 @default.
- W1517822256 hasConceptScore W1517822256C185592680 @default.
- W1517822256 hasConceptScore W1517822256C2778886173 @default.
- W1517822256 hasConceptScore W1517822256C547475151 @default.
- W1517822256 hasConceptScore W1517822256C55493867 @default.
- W1517822256 hasConceptScore W1517822256C71240020 @default.
- W1517822256 hasIssue "10" @default.
- W1517822256 hasLocation W15178222561 @default.
- W1517822256 hasLocation W15178222562 @default.
- W1517822256 hasOpenAccess W1517822256 @default.
- W1517822256 hasPrimaryLocation W15178222561 @default.