FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1519242080> ?p ?o ?g. }

• W1519242080 endingPage "411" @default.
• W1519242080 startingPage "404" @default.
• W1519242080 abstract "1-Methylpyrene (1-MP) is a widespread pollutant that is carcinogenic in animals following metabolic activation. Previous studies have shown that benzylic hydroxylation of 1-MP, catalyzed by multiple CYP isoforms, gives rise to 1-hydroxymethylpyrene (1-HMP), which becomes bioreactive following further metabolism by various sulfotransferase (SULT) isoforms. However, the mutagenic and chromosome damaging effects of 1-MP and 1-HMP in mammalian cells have not been investigated. In this study a Chinese hamster V79-derived cell line expressing both human CYP2E1 and human SULT1A1 was used to investigate the ability of 1-MP and 1-HMP to induce cytotoxicity (using the CCK-8 assay), micronuclei and Hprt gene mutations. The role of each enzyme was investigated through co-exposure in the presence of an enzyme inhibitor. We found that at concentrations of 0.5–4 μM and 5–20 μM, under conditions where no reduction in cell viability/growth occurred, 1-HMP and 1-MP induced micronuclei in V79-hCYP2E1-hSULT1A1 cells in a concentration-dependent manner; however, both compounds were inactive in V79 cells. Similarly, they both caused an increase in Hprt mutant frequency in V79-hCYP2E1-hSULT1A1 cells in these concentration ranges, with 1-MP impairing cell viability/growth at 10 μM and above in the mutagenicity assay. The compounds were again both inactive in V79 cells. The effects of 1-HMP in V79-hCYP2E1-hSULT1A1 cells were blocked or reduced by addition of pentachlorophenol (PCP), a SULT1 inhibitor; the genotoxicity of 1-MP was significantly reduced by either 1-aminobenotrazole, a CYP2E1 inhibitor, or PCP. The results suggest that human CYP2E1 and SULT1A1 cooperate to activate 1-MP and cause genotoxicity in mammalian cells. Environ. Mol. Mutagen. 56:404–411, 2015. © 2014 Wiley Periodicals, Inc." @default.
• W1519242080 created "2016-06-24" @default.
• W1519242080 creator A5012280476 @default.
• W1519242080 creator A5020248084 @default.
• W1519242080 creator A5025289954 @default.
• W1519242080 creator A5029561912 @default.
• W1519242080 creator A5030197645 @default.
• W1519242080 creator A5053382491 @default.
• W1519242080 date "2014-09-22" @default.
• W1519242080 modified "2023-09-24" @default.
• W1519242080 title "Genotoxicity of 1-methylpyrene and 1-hydroxymethylpyrene in chinese hamster V79-derived cells expressing both human CYP2E1 and SULT1A1" @default.
• W1519242080 cites W1978408333 @default.
• W1519242080 cites W1981919438 @default.
• W1519242080 cites W1985374438 @default.
• W1519242080 cites W1985525432 @default.
• W1519242080 cites W1985892587 @default.
• W1519242080 cites W1990665707 @default.
• W1519242080 cites W2005995818 @default.
• W1519242080 cites W2007318225 @default.
• W1519242080 cites W2028881409 @default.
• W1519242080 cites W2030418300 @default.
• W1519242080 cites W2049654926 @default.
• W1519242080 cites W2065747119 @default.
• W1519242080 cites W2074477138 @default.
• W1519242080 cites W2122491364 @default.
• W1519242080 cites W2170089108 @default.
• W1519242080 cites W2172280799 @default.
• W1519242080 doi "https://doi.org/10.1002/em.21912" @default.
• W1519242080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25243916" @default.
• W1519242080 hasPublicationYear "2014" @default.
• W1519242080 type Work @default.
• W1519242080 sameAs 1519242080 @default.
• W1519242080 citedByCount "21" @default.
• W1519242080 countsByYear W15192420802016 @default.
• W1519242080 countsByYear W15192420802017 @default.
• W1519242080 countsByYear W15192420802018 @default.
• W1519242080 countsByYear W15192420802019 @default.
• W1519242080 countsByYear W15192420802020 @default.
• W1519242080 countsByYear W15192420802021 @default.
• W1519242080 countsByYear W15192420802022 @default.
• W1519242080 countsByYear W15192420802023 @default.
• W1519242080 crossrefType "journal-article" @default.
• W1519242080 hasAuthorship W1519242080A5012280476 @default.
• W1519242080 hasAuthorship W1519242080A5020248084 @default.
• W1519242080 hasAuthorship W1519242080A5025289954 @default.
• W1519242080 hasAuthorship W1519242080A5029561912 @default.
• W1519242080 hasAuthorship W1519242080A5030197645 @default.
• W1519242080 hasAuthorship W1519242080A5053382491 @default.
• W1519242080 hasConcept C104317684 @default.
• W1519242080 hasConcept C114246631 @default.
• W1519242080 hasConcept C143065580 @default.
• W1519242080 hasConcept C153911025 @default.
• W1519242080 hasConcept C178790620 @default.
• W1519242080 hasConcept C185592680 @default.
• W1519242080 hasConcept C2777755551 @default.
• W1519242080 hasConcept C2778959862 @default.
• W1519242080 hasConcept C29730261 @default.
• W1519242080 hasConcept C36681516 @default.
• W1519242080 hasConcept C45080847 @default.
• W1519242080 hasConcept C53227056 @default.
• W1519242080 hasConcept C54355233 @default.
• W1519242080 hasConcept C55493867 @default.
• W1519242080 hasConcept C78179603 @default.
• W1519242080 hasConcept C81885089 @default.
• W1519242080 hasConcept C86803240 @default.
• W1519242080 hasConceptScore W1519242080C104317684 @default.
• W1519242080 hasConceptScore W1519242080C114246631 @default.
• W1519242080 hasConceptScore W1519242080C143065580 @default.
• W1519242080 hasConceptScore W1519242080C153911025 @default.
• W1519242080 hasConceptScore W1519242080C178790620 @default.
• W1519242080 hasConceptScore W1519242080C185592680 @default.
• W1519242080 hasConceptScore W1519242080C2777755551 @default.
• W1519242080 hasConceptScore W1519242080C2778959862 @default.
• W1519242080 hasConceptScore W1519242080C29730261 @default.
• W1519242080 hasConceptScore W1519242080C36681516 @default.
• W1519242080 hasConceptScore W1519242080C45080847 @default.
• W1519242080 hasConceptScore W1519242080C53227056 @default.
• W1519242080 hasConceptScore W1519242080C54355233 @default.
• W1519242080 hasConceptScore W1519242080C55493867 @default.
• W1519242080 hasConceptScore W1519242080C78179603 @default.
• W1519242080 hasConceptScore W1519242080C81885089 @default.
• W1519242080 hasConceptScore W1519242080C86803240 @default.
• W1519242080 hasIssue "4" @default.
• W1519242080 hasLocation W15192420801 @default.
• W1519242080 hasLocation W15192420802 @default.
• W1519242080 hasOpenAccess W1519242080 @default.
• W1519242080 hasPrimaryLocation W15192420801 @default.
• W1519242080 hasRelatedWork W1519242080 @default.
• W1519242080 hasRelatedWork W2058530553 @default.
• W1519242080 hasRelatedWork W2082861567 @default.
• W1519242080 hasRelatedWork W2093551617 @default.
• W1519242080 hasRelatedWork W2115118547 @default.
• W1519242080 hasRelatedWork W2126335241 @default.
• W1519242080 hasRelatedWork W2468732756 @default.
• W1519242080 hasRelatedWork W2488298204 @default.
• W1519242080 hasRelatedWork W2988337383 @default.
• W1519242080 hasRelatedWork W3162740924 @default.
• W1519242080 hasVolume "56" @default.

• next