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- W1519271190 abstract "This chapter describes the development and use of a fluorimetric substrate, specific inhibitors, and a polyclonal antiserum directed toward endopeptidase 24.16. These new tools have permitted further investigation of the biochemical properties of the enzyme. It is now possible to monitor the activity in a complex mixture of enzymes such as crude extracts, provided that some precautions are taken. Furthermore, the development of specific inhibitors has established, for the first time, that endopeptidase 24.16 indeed participates in the physiological metabolism of neurotensin. These inhibitors will be of further help in assessing whether the enzyme also contributes to the catabolic pathways of other neuropeptides. Finally, the development of a monospecific antiserum directed toward endopeptidase 24.16 has rendered possible the immunological screening of a rat brain λgt 11 cDNA library and will allow the molecular cloning of the enzyme, which is in progress in the laboratories." @default.
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- W1519271190 date "1995-01-01" @default.
- W1519271190 modified "2023-10-17" @default.
- W1519271190 title "[21] Identification and distribution of endopeptidase 24.16 in central nervous system" @default.
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- W1519271190 doi "https://doi.org/10.1016/s1043-9471(06)80132-1" @default.
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