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- W1519296568 abstract "The pre-steady state kinetics of the hydrolysis of sodium 3-dansylamidomethyl-7-beta (thienyl-2')-acetamidoceph-3-em-4-oate, catalyzed by the beta-lactamase of Staphylococcus aureus PC1, has been studied by the stopped flow method. Fluorescence measurements indicated the presence of two intermediate enzyme-substrate complexes, i.e. at least a three-step mechanism. The disappearance of the characteristic cephem chromophore, which reflects nucleophilic attack at the beta-lactam carbonyl group, correlated with the second step. These results show that an intermediate species with a covalently altered substrate occurs on the reaction path prior to the last step which includes release of product. This intermediate is most likely an acyl-enzyme. The data have been analyzed numerically and the derived rate constants are in accord with the steady state parameters." @default.
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- W1519296568 date "1981-11-01" @default.
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- W1519296568 title "Pre-steady state beta-lactamase kinetics. Observation of a covalent intermediate during turnover of a fluorescent cephalosporin by the beta-lactamase of STaphylococcus aureus PC1." @default.
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- W1519296568 doi "https://doi.org/10.1016/s0021-9258(19)68410-3" @default.
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