FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1519370577> ?p ?o ?g. }

• W1519370577 endingPage "51" @default.
• W1519370577 startingPage "44" @default.
• W1519370577 abstract "Background: BMP-2 (bone morphogenetic protein-2) signals via two types of transmembrane serine/threonine kinase receptors (BRI and BRII), which form heteromeric complexes prior to and after ligand binding. Within a BMP-bound receptor complex, BRII transphosphorylates and activates BRI-a for further signaling. We investigated which signaling pathway is initiated by BMP-2 via preformed receptor complexes versus BMP-2-induced signaling receptor complexes. Methods: Immunofluorescence co-patching was used to study the oligomerization of receptors at the surface of live cells. Binding and chemical cross-linking of iodinated BMP-2 followed by immunoprecipitation was used to show association of receptors in the presence of ligand. Western blots with use of anti-phospho-Smad1 antibodies and reporter gene assays with use of SBE-lux were employed to show activation of the Smad pathway. Phosphorylation of p38-MAPK was shown by Western blots. Induction of alkaline phosphatase was determined by staining the cells. The cluster density of receptors was determined with use of image correlation spectroscopy. Results and Conclusion: We showed that the Smad pathway is induced by preformed receptor complexes, whereas BMP-2-induced signaling complexes result in the activation of p38-MAPK. We also found evidence that the clustering of BRI-a at the membrane is altered in the presence of BRII, suggesting that it associates with existing clusters of BRII to initiate efficient Smad signaling. These data clearly demonstrate that it is critical to fully understand receptor oligomerization in order to estimate signaling outcome for distinct receptor and ligand mutants. Clinical Relevance: The development of BMP-2 antagonists is of special importance for a number of human disorders caused by several members of the BMP/TGF-β (transforming growth factor-beta) superfamily. Since manipulation of BMP-signaling is complex, it is important to understand what influence it might have during the initiation of signaling—:i.e., the oligomerization of BMP receptors to form a signaling receptor complex. There might be cases where either the Smad or the p38 pathway should be targeted." @default.
• W1519370577 created "2016-06-24" @default.
• W1519370577 creator A5004637588 @default.
• W1519370577 creator A5011286984 @default.
• W1519370577 creator A5013661873 @default.
• W1519370577 creator A5022615175 @default.
• W1519370577 creator A5023481028 @default.
• W1519370577 creator A5025036907 @default.
• W1519370577 creator A5044713611 @default.
• W1519370577 creator A5076743435 @default.
• W1519370577 creator A5086554164 @default.
• W1519370577 creator A5087218848 @default.
• W1519370577 date "2003-01-01" @default.
• W1519370577 modified "2023-10-11" @default.
• W1519370577 title "INITIATION OF SMAD-DEPENDENT AND SMAD-INDEPENDENT SIGNALING VIA DISTINCT BMP-RECEPTOR COMPLEXES" @default.
• W1519370577 cites W1589859980 @default.
• W1519370577 cites W1935346479 @default.
• W1519370577 cites W1969944745 @default.
• W1519370577 cites W1971807163 @default.
• W1519370577 cites W1985720487 @default.
• W1519370577 cites W1992374635 @default.
• W1519370577 cites W2003573512 @default.
• W1519370577 cites W2017293256 @default.
• W1519370577 cites W2021628061 @default.
• W1519370577 cites W2039458025 @default.
• W1519370577 cites W2055846324 @default.
• W1519370577 cites W2060041183 @default.
• W1519370577 cites W2061776631 @default.
• W1519370577 cites W2070921472 @default.
• W1519370577 cites W2076439289 @default.
• W1519370577 cites W2079562282 @default.
• W1519370577 cites W2086769750 @default.
• W1519370577 cites W2087988643 @default.
• W1519370577 cites W2090905598 @default.
• W1519370577 cites W2103262612 @default.
• W1519370577 cites W2132008353 @default.
• W1519370577 cites W2132805386 @default.
• W1519370577 doi "https://doi.org/10.2106/00004623-200300003-00009" @default.
• W1519370577 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12925609" @default.
• W1519370577 hasPublicationYear "2003" @default.
• W1519370577 type Work @default.
• W1519370577 sameAs 1519370577 @default.
• W1519370577 citedByCount "105" @default.
• W1519370577 countsByYear W15193705772012 @default.
• W1519370577 countsByYear W15193705772013 @default.
• W1519370577 countsByYear W15193705772014 @default.
• W1519370577 countsByYear W15193705772015 @default.
• W1519370577 countsByYear W15193705772016 @default.
• W1519370577 countsByYear W15193705772017 @default.
• W1519370577 countsByYear W15193705772018 @default.
• W1519370577 countsByYear W15193705772019 @default.
• W1519370577 countsByYear W15193705772020 @default.
• W1519370577 countsByYear W15193705772021 @default.
• W1519370577 countsByYear W15193705772022 @default.
• W1519370577 countsByYear W15193705772023 @default.
• W1519370577 crossrefType "journal-article" @default.
• W1519370577 hasAuthorship W1519370577A5004637588 @default.
• W1519370577 hasAuthorship W1519370577A5011286984 @default.
• W1519370577 hasAuthorship W1519370577A5013661873 @default.
• W1519370577 hasAuthorship W1519370577A5022615175 @default.
• W1519370577 hasAuthorship W1519370577A5023481028 @default.
• W1519370577 hasAuthorship W1519370577A5025036907 @default.
• W1519370577 hasAuthorship W1519370577A5044713611 @default.
• W1519370577 hasAuthorship W1519370577A5076743435 @default.
• W1519370577 hasAuthorship W1519370577A5086554164 @default.
• W1519370577 hasAuthorship W1519370577A5087218848 @default.
• W1519370577 hasConcept C104317684 @default.
• W1519370577 hasConcept C11960822 @default.
• W1519370577 hasConcept C153911025 @default.
• W1519370577 hasConcept C170493617 @default.
• W1519370577 hasConcept C185592680 @default.
• W1519370577 hasConcept C2781080636 @default.
• W1519370577 hasConcept C31507581 @default.
• W1519370577 hasConcept C55493867 @default.
• W1519370577 hasConcept C57074206 @default.
• W1519370577 hasConcept C62478195 @default.
• W1519370577 hasConcept C73834784 @default.
• W1519370577 hasConcept C86803240 @default.
• W1519370577 hasConcept C95444343 @default.
• W1519370577 hasConceptScore W1519370577C104317684 @default.
• W1519370577 hasConceptScore W1519370577C11960822 @default.
• W1519370577 hasConceptScore W1519370577C153911025 @default.
• W1519370577 hasConceptScore W1519370577C170493617 @default.
• W1519370577 hasConceptScore W1519370577C185592680 @default.
• W1519370577 hasConceptScore W1519370577C2781080636 @default.
• W1519370577 hasConceptScore W1519370577C31507581 @default.
• W1519370577 hasConceptScore W1519370577C55493867 @default.
• W1519370577 hasConceptScore W1519370577C57074206 @default.
• W1519370577 hasConceptScore W1519370577C62478195 @default.
• W1519370577 hasConceptScore W1519370577C73834784 @default.
• W1519370577 hasConceptScore W1519370577C86803240 @default.
• W1519370577 hasConceptScore W1519370577C95444343 @default.
• W1519370577 hasLocation W15193705771 @default.
• W1519370577 hasLocation W15193705772 @default.
• W1519370577 hasOpenAccess W1519370577 @default.
• W1519370577 hasPrimaryLocation W15193705771 @default.
• W1519370577 hasRelatedWork W1521595843 @default.
• W1519370577 hasRelatedWork W1558754969 @default.

• next