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- W1519422036 abstract "Publisher Summary This chapter discusses the elutriation of budding yeast. To synchronize yeast by elutriation, a population of exponential phase cells is fractionated on the basis of size using counterflow centrifugation. With budding yeast a fraction of small unbudded G1 cells is usually harvested in fresh medium and when successfully carried out, outgrowth of these cells provides synchrony over at least two cell cycles. In a number of circumstances, elutriation is particularly useful. Thus elutriation provides an essential means of synchronization under particular circumstances and can also be used to establish relatively artifact-free cultures. This chapter describes the principle and provides detailed protocols for the procedure. Centrifugal elutriation depends on two independent means of separating cells. The first is straightforward centrifugal sedimentation, while the second is elutriation, whereby an appropriate fluid is pumped through the elutriation chamber from the bottom, the narrow end, thus directly opposing the centrifugal force." @default.
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- W1519422036 date "1997-01-01" @default.
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- W1519422036 title "Elutriation of budding yeast" @default.
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- W1519422036 doi "https://doi.org/10.1016/s0076-6879(97)83028-5" @default.
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