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• W1519473917 abstract "Herpes simplex virus (HSV) entry requires four essential glycoproteins (gD, gH/gL, and gB) to enable fusion between the virion envelope and the cellular membrane. The fusion cascade is activated by gD binding to one of its receptors, nectin-1 or HVEM. Glycoprotein B (gB), a class III viral fusion protein, mediates the fusion reaction, while data indicates that gH/gL acts as a regulator of gB. gB is trimeric and has a 773 amino acid ectodomain with a highly hydrophobic membrane proximal region (MPR) (residues 731-773) and two fusion loops (FL) per protomer. The post-fusion structure of gB was solved from the gB(730t) construct, which is truncated to remove the hydrophobic MPR residues. In this dissertation I investigated the MPRs influence on gBs ability to interact with membranes. I hypothesize that the MPR regulates fusion loop exposure by interacting with the fusion loops and masks them until fusion begins. To investigate this process I constructed a series of MPR deletion, truncation, and point mutations using both full-length mammalian expression vectors and purified baculovirus expressed protein. I found that deletions in the MPR from full-length gB resulted in a disruption in cell surface expression in transfected cells. This suggests the MPR is necessary for proper folding or transport of gB. Soluble gB MPR truncations [gB(759t), gB(749t), gB(739t)] were expressed and purified using the baculovirus expression system, and compared to MPR-less gB(730t) and full MPR containing gB(773t). I found that gB containing an MPR segment were all compromised in their ability to bind liposomes in comparison to gB(730t), which lacks any MPR residues. Supporting our hypothesis we found that residues 731 to 739 were sufficient prevent liposome association and mutation of two aromatic residues, F732 and F739, to alanine in gB(739t) restored gBs ability to bind liposomes. Together, my data suggests the MPR does indeed regulate gBs ability to associate with liposomes, and that aromatic residues in the MPR are important for this function. This supports our model that the MPR masks the gB FLs to prevent premature membrane association and adds another layer of regulation to the HSV entry cascade." @default.
• W1519473917 created "2016-06-24" @default.
• W1519473917 creator A5052923962 @default.
• W1519473917 date "2013-01-01" @default.
• W1519473917 modified "2023-09-27" @default.
• W1519473917 title "Defining A Regulatory Role For The Hsv Glycoprotein B Membrane Proximal Region In Membrane Association" @default.
• W1519473917 cites W1485800319 @default.
• W1519473917 cites W1488293738 @default.
• W1519473917 cites W1492231753 @default.
• W1519473917 cites W1523114181 @default.
• W1519473917 cites W1528872128 @default.
• W1519473917 cites W1544234519 @default.
• W1519473917 cites W1582131845 @default.
• W1519473917 cites W1594175085 @default.
• W1519473917 cites W1606664220 @default.
• W1519473917 cites W1675610649 @default.
• W1519473917 cites W1780295430 @default.
• W1519473917 cites W1811042257 @default.
• W1519473917 cites W1849416217 @default.
• W1519473917 cites W1884766802 @default.
• W1519473917 cites W1905880865 @default.
• W1519473917 cites W1937443703 @default.
• W1519473917 cites W1962945032 @default.
• W1519473917 cites W1967325223 @default.
• W1519473917 cites W1969376060 @default.
• W1519473917 cites W1973784039 @default.
• W1519473917 cites W1975304761 @default.
• W1519473917 cites W1977767269 @default.
• W1519473917 cites W1978377488 @default.
• W1519473917 cites W1978751572 @default.
• W1519473917 cites W1978763290 @default.
• W1519473917 cites W1979255492 @default.
• W1519473917 cites W1979385877 @default.
• W1519473917 cites W1980953487 @default.
• W1519473917 cites W1981195949 @default.
• W1519473917 cites W1984085926 @default.
• W1519473917 cites W1984412128 @default.
• W1519473917 cites W1985339853 @default.
• W1519473917 cites W1985422811 @default.
• W1519473917 cites W1986015652 @default.
• W1519473917 cites W1988071861 @default.
• W1519473917 cites W1988157771 @default.
• W1519473917 cites W1988327539 @default.
• W1519473917 cites W1988723707 @default.
• W1519473917 cites W1989637234 @default.
• W1519473917 cites W1992007669 @default.
• W1519473917 cites W1992659717 @default.
• W1519473917 cites W1993001714 @default.
• W1519473917 cites W1993703438 @default.
• W1519473917 cites W1994823455 @default.
• W1519473917 cites W1995181927 @default.
• W1519473917 cites W1996795546 @default.
• W1519473917 cites W1996990741 @default.
• W1519473917 cites W1997221411 @default.
• W1519473917 cites W1997505475 @default.
• W1519473917 cites W1997725738 @default.
• W1519473917 cites W2001371234 @default.
• W1519473917 cites W2002193732 @default.
• W1519473917 cites W2002671791 @default.
• W1519473917 cites W2003879839 @default.
• W1519473917 cites W2004773897 @default.
• W1519473917 cites W2008486572 @default.
• W1519473917 cites W2009929706 @default.
• W1519473917 cites W2012451546 @default.
• W1519473917 cites W2012922169 @default.
• W1519473917 cites W2013607556 @default.
• W1519473917 cites W2014568747 @default.
• W1519473917 cites W2015161652 @default.
• W1519473917 cites W2015829412 @default.
• W1519473917 cites W2019203713 @default.
• W1519473917 cites W2019500839 @default.
• W1519473917 cites W2021490026 @default.
• W1519473917 cites W2021968536 @default.
• W1519473917 cites W2024965784 @default.
• W1519473917 cites W2026390603 @default.
• W1519473917 cites W2026563152 @default.
• W1519473917 cites W2026870663 @default.
• W1519473917 cites W2027657241 @default.
• W1519473917 cites W2027929104 @default.
• W1519473917 cites W2029459850 @default.
• W1519473917 cites W2031152855 @default.
• W1519473917 cites W2032966566 @default.
• W1519473917 cites W2033096908 @default.
• W1519473917 cites W2034172998 @default.
• W1519473917 cites W2035233205 @default.
• W1519473917 cites W2035655655 @default.
• W1519473917 cites W2039451739 @default.
• W1519473917 cites W2040441008 @default.
• W1519473917 cites W2041055896 @default.
• W1519473917 cites W2041994321 @default.
• W1519473917 cites W2042260827 @default.
• W1519473917 cites W2043074722 @default.
• W1519473917 cites W2044142593 @default.
• W1519473917 cites W2044243584 @default.
• W1519473917 cites W2044847443 @default.
• W1519473917 cites W2045139191 @default.
• W1519473917 cites W2045748011 @default.
• W1519473917 cites W2046254711 @default.
• W1519473917 cites W2047683017 @default.
• W1519473917 cites W2047810146 @default.

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