Matches in SemOpenAlex for { <https://semopenalex.org/work/W1519820315> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1519820315 endingPage "137" @default.
- W1519820315 startingPage "125" @default.
- W1519820315 abstract "1. Cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) was purified from the mycelial fungus Botryodiplodia theobromae by sodium cholate and ammonium sulfate solubilization, ammonium sulfate fractionation, and DEAE-cellulose chromatography. The purified enzyme contained 6--7 nmol heme a/mg of protein. The specific activity of the purified enzyme was 2.1--2.3 . 10(3) k (min-1) per mg of protein with 15 mumol ferrocytochrome c and at pH 5.9 and optimal phosphate and Tween 80 concentrations (65 mM and 0.1%, respectively). The Km for ferrocytochrome c was determined to be 1.2--1.3 . 10(-5) M, while at infinite substrate concentration the enzyme catalyzed the oxidation of about 60 mumole of ferrocytochrome c/min per mg of protein. Sedimentation behavior and kinetic evidence suggest that the purified enzyme exists as aggregates of the single molecule. The purified B. theobromae cytochrome c oxidase with its 428 nm Soret absorption maximum may be similar if not identical to oxygenated forms of the enzyme from other fungal species. 2. The purified enzyme was shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis to consist of seven polypeptides with the following respective molecular weights: I, 41 000; II, 28 000; III, 19 000; IV, 14 800; V, 12 800; VI, 11 500; and VII, 9300. Biosynthesis studies showed that the three highest molecular weight polypeptides of the enzyme were synthesized on mitochondrial ribosomes, and the four smaller polypeptides were products of cytoplasmic ribosomes." @default.
- W1519820315 created "2016-06-24" @default.
- W1519820315 creator A5023994814 @default.
- W1519820315 creator A5054683540 @default.
- W1519820315 date "1980-01-01" @default.
- W1519820315 modified "2023-09-27" @default.
- W1519820315 title "Mitochondrial biogenesis during fungal spore germination" @default.
- W1519820315 cites W1266496976 @default.
- W1519820315 cites W1482425831 @default.
- W1519820315 cites W1501711074 @default.
- W1519820315 cites W1505840681 @default.
- W1519820315 cites W1520290682 @default.
- W1519820315 cites W1529135680 @default.
- W1519820315 cites W1541569010 @default.
- W1519820315 cites W1552792204 @default.
- W1519820315 cites W1553938468 @default.
- W1519820315 cites W1556421275 @default.
- W1519820315 cites W1579538747 @default.
- W1519820315 cites W1604320886 @default.
- W1519820315 cites W1642939127 @default.
- W1519820315 cites W1775749144 @default.
- W1519820315 cites W1931456200 @default.
- W1519820315 cites W1968319193 @default.
- W1519820315 cites W1971793869 @default.
- W1519820315 cites W1978393565 @default.
- W1519820315 cites W1983668914 @default.
- W1519820315 cites W1987461561 @default.
- W1519820315 cites W1995710377 @default.
- W1519820315 cites W2040395881 @default.
- W1519820315 cites W2069182758 @default.
- W1519820315 cites W2079938883 @default.
- W1519820315 cites W2098795844 @default.
- W1519820315 cites W2132568098 @default.
- W1519820315 cites W2139281011 @default.
- W1519820315 cites W2148487315 @default.
- W1519820315 cites W2159067289 @default.
- W1519820315 cites W2175651785 @default.
- W1519820315 cites W2316486410 @default.
- W1519820315 cites W2332125373 @default.
- W1519820315 cites W2409378880 @default.
- W1519820315 cites W4255726880 @default.
- W1519820315 doi "https://doi.org/10.1016/0005-2787(80)90104-5" @default.
- W1519820315 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6243487" @default.
- W1519820315 hasPublicationYear "1980" @default.
- W1519820315 type Work @default.
- W1519820315 sameAs 1519820315 @default.
- W1519820315 citedByCount "6" @default.
- W1519820315 crossrefType "journal-article" @default.
- W1519820315 hasAuthorship W1519820315A5023994814 @default.
- W1519820315 hasAuthorship W1519820315A5054683540 @default.
- W1519820315 hasConcept C101660505 @default.
- W1519820315 hasConcept C116084860 @default.
- W1519820315 hasConcept C123249941 @default.
- W1519820315 hasConcept C181199279 @default.
- W1519820315 hasConcept C185592680 @default.
- W1519820315 hasConcept C2778913249 @default.
- W1519820315 hasConcept C2779268744 @default.
- W1519820315 hasConcept C43617362 @default.
- W1519820315 hasConcept C55493867 @default.
- W1519820315 hasConcept C58121356 @default.
- W1519820315 hasConceptScore W1519820315C101660505 @default.
- W1519820315 hasConceptScore W1519820315C116084860 @default.
- W1519820315 hasConceptScore W1519820315C123249941 @default.
- W1519820315 hasConceptScore W1519820315C181199279 @default.
- W1519820315 hasConceptScore W1519820315C185592680 @default.
- W1519820315 hasConceptScore W1519820315C2778913249 @default.
- W1519820315 hasConceptScore W1519820315C2779268744 @default.
- W1519820315 hasConceptScore W1519820315C43617362 @default.
- W1519820315 hasConceptScore W1519820315C55493867 @default.
- W1519820315 hasConceptScore W1519820315C58121356 @default.
- W1519820315 hasIssue "1" @default.
- W1519820315 hasLocation W15198203151 @default.
- W1519820315 hasLocation W15198203152 @default.
- W1519820315 hasOpenAccess W1519820315 @default.
- W1519820315 hasPrimaryLocation W15198203151 @default.
- W1519820315 hasRelatedWork W1440031586 @default.
- W1519820315 hasRelatedWork W1981694126 @default.
- W1519820315 hasRelatedWork W2033350752 @default.
- W1519820315 hasRelatedWork W2033749400 @default.
- W1519820315 hasRelatedWork W2039541255 @default.
- W1519820315 hasRelatedWork W2067099198 @default.
- W1519820315 hasRelatedWork W2074738418 @default.
- W1519820315 hasRelatedWork W2126246256 @default.
- W1519820315 hasRelatedWork W2161798594 @default.
- W1519820315 hasRelatedWork W2463802760 @default.
- W1519820315 hasVolume "606" @default.
- W1519820315 isParatext "false" @default.
- W1519820315 isRetracted "false" @default.
- W1519820315 magId "1519820315" @default.
- W1519820315 workType "article" @default.