Matches in SemOpenAlex for { <https://semopenalex.org/work/W1520079972> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1520079972 endingPage "4520" @default.
- W1520079972 startingPage "4516" @default.
- W1520079972 abstract "Abstract The role of plasma membrane Cl(-)-HCO-3-exchange in regulating intracellular pH (pHi) was examined in Madin-Darby canine kidney cell monolayers. In cells bathed in 25 mM HCO-3, pH 7.4, steady state pHi was 7.10 +/- 0.03 (n = 14) measured with the fluorescent pH probe 2',7'-biscarboxyethyl-5,6-carboxyfluorescein. Following acute alkaline loading, pHi recovered exponentially in approximately 4 min. The recovery rate was significantly decreased by Cl- or HCO-3 removal and in the presence of 50 microM 4,4'-diisothiocyano-2,2'-disulfonic stilbene (DIDS). Na+ removal or 10(-3) M amiloride did not inhibit the pHi recovery rate after an acute alkaline load. Following acute intracellular acidification, the pHi recovery rate was significantly inhibited by 10(-3) M amiloride but was not altered by Cl- removal or 50 microM DIDS. At an extracellular pH (pHo) of 7.4, pHi remained unchanged when the cells were bathed in either Cl- free media, HCO-3 free media, or in the presence of 50 microM DIDS. As pHo was increased to 8.0, steady state pHi was significantly greater than control in Cl(-)-free media and in the presence of 50 microM DIDS. It is concluded that Madin-Darby canine kidney cells possess a Na+-independent Cl(-)-HCO-3 exchanger with a Km for external Cl- of approximately 6 mM. The exchanger plays an important role in pHi regulation following an elevation of pHi above approximately 7.1. Recovery of pHi following intracellular acidification is mediated by the Na+/H+ antiporter and not the anion exchanger." @default.
- W1520079972 created "2016-06-24" @default.
- W1520079972 creator A5024291887 @default.
- W1520079972 creator A5048085772 @default.
- W1520079972 date "1987-04-01" @default.
- W1520079972 modified "2023-10-15" @default.
- W1520079972 title "Na+-independent Cl(-)-HCO-3- exchange in Madin-Darby canine kidney cells. Role in intracellular pH regulation." @default.
- W1520079972 cites W140004033 @default.
- W1520079972 cites W1491178385 @default.
- W1520079972 cites W1505021935 @default.
- W1520079972 cites W1570821412 @default.
- W1520079972 cites W1601376839 @default.
- W1520079972 cites W1973058160 @default.
- W1520079972 cites W1978399382 @default.
- W1520079972 cites W1990659240 @default.
- W1520079972 cites W1996699109 @default.
- W1520079972 cites W2026099323 @default.
- W1520079972 cites W2072235172 @default.
- W1520079972 cites W2106973449 @default.
- W1520079972 cites W2107856629 @default.
- W1520079972 cites W2140994830 @default.
- W1520079972 cites W2151041721 @default.
- W1520079972 cites W2151703154 @default.
- W1520079972 cites W2158699967 @default.
- W1520079972 cites W2163129250 @default.
- W1520079972 cites W2163484228 @default.
- W1520079972 cites W2404138573 @default.
- W1520079972 cites W2410446840 @default.
- W1520079972 cites W4238605098 @default.
- W1520079972 doi "https://doi.org/10.1016/s0021-9258(18)61222-0" @default.
- W1520079972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3558351" @default.
- W1520079972 hasPublicationYear "1987" @default.
- W1520079972 type Work @default.
- W1520079972 sameAs 1520079972 @default.
- W1520079972 citedByCount "88" @default.
- W1520079972 crossrefType "journal-article" @default.
- W1520079972 hasAuthorship W1520079972A5024291887 @default.
- W1520079972 hasAuthorship W1520079972A5048085772 @default.
- W1520079972 hasBestOaLocation W15200799721 @default.
- W1520079972 hasConcept C114574056 @default.
- W1520079972 hasConcept C12554922 @default.
- W1520079972 hasConcept C134018914 @default.
- W1520079972 hasConcept C134897140 @default.
- W1520079972 hasConcept C178790620 @default.
- W1520079972 hasConcept C185592680 @default.
- W1520079972 hasConcept C2780091579 @default.
- W1520079972 hasConcept C537181965 @default.
- W1520079972 hasConcept C55493867 @default.
- W1520079972 hasConcept C79879829 @default.
- W1520079972 hasConcept C86803240 @default.
- W1520079972 hasConcept C95444343 @default.
- W1520079972 hasConceptScore W1520079972C114574056 @default.
- W1520079972 hasConceptScore W1520079972C12554922 @default.
- W1520079972 hasConceptScore W1520079972C134018914 @default.
- W1520079972 hasConceptScore W1520079972C134897140 @default.
- W1520079972 hasConceptScore W1520079972C178790620 @default.
- W1520079972 hasConceptScore W1520079972C185592680 @default.
- W1520079972 hasConceptScore W1520079972C2780091579 @default.
- W1520079972 hasConceptScore W1520079972C537181965 @default.
- W1520079972 hasConceptScore W1520079972C55493867 @default.
- W1520079972 hasConceptScore W1520079972C79879829 @default.
- W1520079972 hasConceptScore W1520079972C86803240 @default.
- W1520079972 hasConceptScore W1520079972C95444343 @default.
- W1520079972 hasIssue "10" @default.
- W1520079972 hasLocation W15200799721 @default.
- W1520079972 hasOpenAccess W1520079972 @default.
- W1520079972 hasPrimaryLocation W15200799721 @default.
- W1520079972 hasRelatedWork W2014477529 @default.
- W1520079972 hasRelatedWork W2051540329 @default.
- W1520079972 hasRelatedWork W2055659392 @default.
- W1520079972 hasRelatedWork W2056895997 @default.
- W1520079972 hasRelatedWork W2144332423 @default.
- W1520079972 hasRelatedWork W2163342739 @default.
- W1520079972 hasRelatedWork W2394514550 @default.
- W1520079972 hasRelatedWork W2394522925 @default.
- W1520079972 hasRelatedWork W2462160267 @default.
- W1520079972 hasRelatedWork W600556144 @default.
- W1520079972 hasVolume "262" @default.
- W1520079972 isParatext "false" @default.
- W1520079972 isRetracted "false" @default.
- W1520079972 magId "1520079972" @default.
- W1520079972 workType "article" @default.