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- W1520225081 abstract "Abstract Different amino acid substitutions were generated at positions 3 and 61 in the lac repressor polypeptide chain by the suppression and reversion of nonsense mutations. Tyrosine in place of proline at position 3 results in a repressor which binds lac operator 100 times more tightly than wild-type repressor. Binding to DNA not containing the lac operator is also increased. A similar effect is seen for the serine to leucine exchange at position 61, which recreates the repressor resulting from a previously characterized missense mutation, X86 (Chamness & Willson, 1970; Jobe & Bourgeois, 1972; Appendix to this paper). Tyrosine in place of serine at position 61 also causes a tight binding effect, but not as strong as in the case of the leucine replacement. A repressor containing tyrosine at position 3 and leucine at position 61 resulted from the construction of the appropriate double mutant. This repressor binds both operator and non-operator DNA extremely tightly, the affinities being increased approximately 10,000-fold over wild-type. The binding to non-operator DNA (such as λ DNA) is sensitive to isopropyl-β, d -thiogalactoside." @default.
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- W1520225081 date "1978-08-01" @default.
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- W1520225081 title "Genetic studies of the lac repressor" @default.
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- W1520225081 doi "https://doi.org/10.1016/0022-2836(78)90089-x" @default.
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