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- W1520435948 abstract "We developed cryo-scanning x-ray diffraction microscopy, utilizing hard x-ray ptychography at cryogenic temperature, for the noninvasive, high-resolution imaging of wet, extended biological samples and report its first frozen-hydrated imaging. Utilizing phase contrast at hard x-rays, cryo-scanning x-ray diffraction microscopy provides the penetration power suitable for thick samples while retaining sensitivity to minute density changes within unstained samples. It is dose-efficient and further minimizes radiation damage by keeping the wet samples at cryogenic temperature. We demonstrate these capabilities in two dimensions by imaging unstained frozen-hydrated budding yeast cells, achieving a spatial resolution of 85 nm with a phase sensitivity of 0.0053 radians. The current work presents the feasibility of cryo-scanning x-ray diffraction microscopy for quantitative, high-resolution imaging of unmodified biological samples extending to tens of micrometres." @default.
- W1520435948 created "2016-06-24" @default.
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- W1520435948 date "2012-12-10" @default.
- W1520435948 modified "2023-10-16" @default.
- W1520435948 title "Cryo-scanning x-ray diffraction microscopy of frozen-hydrated yeast" @default.
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- W1520435948 doi "https://doi.org/10.1111/j.1365-2818.2012.03682.x" @default.
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