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- W1520443578 abstract "The fractionation of human plasma by chromatography on immobilized Brown Fractogel TSK-AF was analyzed by following the elution profile of 25 different plasma proteins. A three-step procedure was used to elute proteins from the column. First, a low-molarity buffer (30 mM sodium phosphate, pH 7.0, I = 0.053) was applied; then a linear salt gradient (0-1.0 M sodium chloride in the above buffer) was followed by an additional wash with four bed volumes of 1.0 M sodium chloride. Tightly bound proteins were finally stripped with 0.5 M ammonium thiocyanate. The elution profile of the proteins obtained by this procedure appears to be very reproducible. Comparison with the profiles obtained by chromatography on Cibacron Blue 3-GA and on Green TSK-AF indicates significant differences between the binding properties of the three gels." @default.
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- W1520443578 date "1987-06-01" @default.
- W1520443578 modified "2023-09-26" @default.
- W1520443578 title "Fractionation of plasma proteins by pseudo-ligand affinity chromatography" @default.
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- W1520443578 doi "https://doi.org/10.1016/s0021-9673(01)85002-0" @default.
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