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- W1520455089 abstract "Publisher Summary This chapter describes methods utilized to identify and purify three different types of gene-specific transcription factors. Studies of gene expression in bacterial and eukaryotic organisms, as well as the viruses that infect them, have identified two broad categories of transcription factors, including ones that are generally utilized for the expression of all or most genes within an organism and those that are gene specific. Prototypical of the former category are the RNA polymerases. Gene-specific transcription factors are, by definition, utilized to regulate the expression of a limited set of genes within a cell or organism. Genes are selectively expressed according to their interplay with gene-specific transcription factors. A general property of these proteins includes the capacity to bind avidly and selectively to relatively short sequences of DNA (6 to 12 bp in length). If a gene contains a binding site for such a factor, so long as the site is appropriately disposed relative to other regulatory components of the gene, it is endowed responsive to that factor. As such, the presence or absence of the relevant factor can govern the expression of that gene. If a gene does not contain such a binding site, its expression is, thereby not directly influenced by the factor. Methods have been developed to allow rapid isolation and purification of gene-specific transcription factors." @default.
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- W1520455089 date "1996-01-01" @default.
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- W1520455089 title "Purification and characterization of gene-specific transcription factors: , GABP, and IL-4 stat" @default.
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- W1520455089 doi "https://doi.org/10.1016/s0076-6879(96)74010-7" @default.
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