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- W1520481055 abstract "Publisher Summary The obvious advantages of the chick for embryological studies have been tempered by the difficulties of genetic analyses in this organism. The ability to manipulate gene expression in the mouse embryo has rendered it the organism of choice for genetic studies, but the difficulty of physically manipulating the mammalian embryo limits the combination of these approaches to study development. The use of retroviral vectors to alter gene expression has proven to be a valuable alternative to transgenic approaches. Many of the genetic manipulations that have proven so powerful in the mouse can be mimicked with retroviral vectors. The use of retroviruses to alter gene expression has several advantages stemming from the comparative ease with which different expression patterns may be generated with the same virus. Many genes are reused in different tissues and at different times to mediate development. By varying the infection protocol, a single high titer virus stock may be used to address the role of a gene in many different developmental decisions." @default.
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- W1520481055 date "1996-01-01" @default.
- W1520481055 modified "2023-10-12" @default.
- W1520481055 title "Chapter 10 Manipulating Gene Expression with Replication--Competent Retroviruses" @default.
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- W1520481055 doi "https://doi.org/10.1016/s0091-679x(08)60629-9" @default.
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