Matches in SemOpenAlex for { <https://semopenalex.org/work/W1520578824> ?p ?o ?g. }
- W1520578824 endingPage "2754" @default.
- W1520578824 startingPage "2748" @default.
- W1520578824 abstract "The Neurospora crassa metallothionein (NC) synthesis gene was cloned and expressed in Escherichia coli in two different expression vectors (pING2 and pUA7), both under the regulation of the Salmonella typhimurium arabinose operon. Upon induction with arabinose, the pING2-NC vector expressed as inclusion body-localized AraB'::NC fusion protein of 21 kilodaltons. The pUA7-NC vector expressed a 5.3-kilodalton Lpp::NC fusion protein anchored to the outer membrane of the cell. Cells expressing the NC fusion proteins accumulated Cd2+ and Cu+ (between 2.3- and 11-fold) compared with nonexpressing cells. To generate novel forms of metal-binding peptides, a set of specific mutant genes for N. crassa NC was designed in which each cysteine residue was replaced with a subset of amino acids implicated in peptide-metal coordination (Asn, Asp, His, Lys, or Tyr residues). These mutant NC sequences were cloned into the two vectors and expressed in E. coli. One of the mutant proteins (containing His residues) showed accumulation of Cd2+ and Cu+ (threefold) from a mixture of 16 heavy metals species. None of the other heavy metals present in the culture was accumulated." @default.
- W1520578824 created "2016-06-24" @default.
- W1520578824 creator A5045461476 @default.
- W1520578824 creator A5060087834 @default.
- W1520578824 creator A5086479845 @default.
- W1520578824 date "1990-09-01" @default.
- W1520578824 modified "2023-10-16" @default.
- W1520578824 title "Expression of a Neurospora crassa metallothionein and its variants in Escherichia coli" @default.
- W1520578824 cites W131069573 @default.
- W1520578824 cites W1490206767 @default.
- W1520578824 cites W149789775 @default.
- W1520578824 cites W1556454508 @default.
- W1520578824 cites W1948309971 @default.
- W1520578824 cites W1975085160 @default.
- W1520578824 cites W2003077115 @default.
- W1520578824 cites W2007493406 @default.
- W1520578824 cites W2010719546 @default.
- W1520578824 cites W2015894694 @default.
- W1520578824 cites W2018150819 @default.
- W1520578824 cites W2020551760 @default.
- W1520578824 cites W2022338802 @default.
- W1520578824 cites W2023269562 @default.
- W1520578824 cites W2028473313 @default.
- W1520578824 cites W2032458072 @default.
- W1520578824 cites W2054491767 @default.
- W1520578824 cites W2058349098 @default.
- W1520578824 cites W2061791653 @default.
- W1520578824 cites W2064470652 @default.
- W1520578824 cites W2100837269 @default.
- W1520578824 cites W2109068357 @default.
- W1520578824 cites W2138270253 @default.
- W1520578824 cites W2149122019 @default.
- W1520578824 cites W2161524167 @default.
- W1520578824 cites W2165766524 @default.
- W1520578824 cites W2469775366 @default.
- W1520578824 cites W4211008517 @default.
- W1520578824 cites W4211216890 @default.
- W1520578824 cites W4250221563 @default.
- W1520578824 doi "https://doi.org/10.1128/aem.56.9.2748-2754.1990" @default.
- W1520578824 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/184837" @default.
- W1520578824 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2148862" @default.
- W1520578824 hasPublicationYear "1990" @default.
- W1520578824 type Work @default.
- W1520578824 sameAs 1520578824 @default.
- W1520578824 citedByCount "27" @default.
- W1520578824 countsByYear W15205788242012 @default.
- W1520578824 countsByYear W15205788242014 @default.
- W1520578824 countsByYear W15205788242018 @default.
- W1520578824 crossrefType "journal-article" @default.
- W1520578824 hasAuthorship W1520578824A5045461476 @default.
- W1520578824 hasAuthorship W1520578824A5060087834 @default.
- W1520578824 hasAuthorship W1520578824A5086479845 @default.
- W1520578824 hasBestOaLocation W15205788241 @default.
- W1520578824 hasConcept C104317684 @default.
- W1520578824 hasConcept C123894998 @default.
- W1520578824 hasConcept C143065580 @default.
- W1520578824 hasConcept C153911025 @default.
- W1520578824 hasConcept C203075996 @default.
- W1520578824 hasConcept C20719415 @default.
- W1520578824 hasConcept C2776739539 @default.
- W1520578824 hasConcept C2776769606 @default.
- W1520578824 hasConcept C2777251434 @default.
- W1520578824 hasConcept C33161422 @default.
- W1520578824 hasConcept C40767141 @default.
- W1520578824 hasConcept C515207424 @default.
- W1520578824 hasConcept C547475151 @default.
- W1520578824 hasConcept C55493867 @default.
- W1520578824 hasConcept C86803240 @default.
- W1520578824 hasConceptScore W1520578824C104317684 @default.
- W1520578824 hasConceptScore W1520578824C123894998 @default.
- W1520578824 hasConceptScore W1520578824C143065580 @default.
- W1520578824 hasConceptScore W1520578824C153911025 @default.
- W1520578824 hasConceptScore W1520578824C203075996 @default.
- W1520578824 hasConceptScore W1520578824C20719415 @default.
- W1520578824 hasConceptScore W1520578824C2776739539 @default.
- W1520578824 hasConceptScore W1520578824C2776769606 @default.
- W1520578824 hasConceptScore W1520578824C2777251434 @default.
- W1520578824 hasConceptScore W1520578824C33161422 @default.
- W1520578824 hasConceptScore W1520578824C40767141 @default.
- W1520578824 hasConceptScore W1520578824C515207424 @default.
- W1520578824 hasConceptScore W1520578824C547475151 @default.
- W1520578824 hasConceptScore W1520578824C55493867 @default.
- W1520578824 hasConceptScore W1520578824C86803240 @default.
- W1520578824 hasIssue "9" @default.
- W1520578824 hasLocation W15205788241 @default.
- W1520578824 hasLocation W15205788242 @default.
- W1520578824 hasLocation W15205788243 @default.
- W1520578824 hasOpenAccess W1520578824 @default.
- W1520578824 hasPrimaryLocation W15205788241 @default.
- W1520578824 hasRelatedWork W1553937872 @default.
- W1520578824 hasRelatedWork W2031718479 @default.
- W1520578824 hasRelatedWork W2048070982 @default.
- W1520578824 hasRelatedWork W2051650982 @default.
- W1520578824 hasRelatedWork W2112954302 @default.
- W1520578824 hasRelatedWork W2113665404 @default.
- W1520578824 hasRelatedWork W2122922002 @default.
- W1520578824 hasRelatedWork W2135385723 @default.
- W1520578824 hasRelatedWork W2418080363 @default.