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• W1520580141 abstract "The extent of homology between herpes simplex virus 1 and 2 (HSV-1 and HSV-2) deoxyribonucleic acid (DNA) was measured in two ways: (i) by determination of the relative rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA immobilized on filters and (ii) by determination of the rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA in solution. Approximately 40% of HSV-1 and HSV-2 DNA is homologous at hybridization temperatures 25 C below the melting temperature (T m ) of HSV DNA (liquid-filter annealing). Lowering the temperature to 34 C below the T m increased the extent of homology to 46% (liquid annealing). The extent of base-pairing in HSV-1-HSV-2 heteroduplex DNA was determined by thermal chromatography on hydroxyapatite. Heteroduplexes of HSV-1 and HSV-2 DNA eluted in a single peak whose midpoint (Te 50 ) was 10 C below that of the homoduplex. Conspicuously absent were heteroduplexes that eluted at more than 15 C below the Te 50 of the homoduplex. The data indicate the existence of a variable region of DNA (54%) with very little, if any, homology and an invariable region (46%) with relatively good (85%) matching of base pairs." @default.
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• W1520580141 date "1972-05-01" @default.
• W1520580141 modified "2023-10-03" @default.
• W1520580141 title "Genetic Relatedness of Type 1 and Type 2 Herpes Simplex Viruses" @default.
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• W1520580141 doi "https://doi.org/10.1128/jvi.9.5.738-745.1972" @default.
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