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• W1520592032 abstract "Abstract The number of molecules of C3 on human RBC was determined by the method of Borsos and Leonard (J. Immunol., 107: 766, 1971) based on inhibition of anti-C3 antibody by cell-bound C3. The assay was standardized with fluid phase C3 of known concentration and with RBC sensitized with radiolabeled C3. Normal RBC and cells sensitized with IgG gave negative results. Lewis antibody was used to sensitize RBC with C3 in vitro. In vivo sensitized RBC were obtained from 25 patients having a positive anti-C3 antiglobulin test. The antiglobulin test with anti-C3 antiglobulin serum was weakly positive with 115 molecules C3/RBC, and was strongly positive with &gt;1000 molecules C3/RBC. There was a good correlation between antiglobulin test titration score and number of molecules C3/RBC, but for a given score, there were about three times as many C3 molecules/RBC on in vitro vs. in vivo sensitized cells." @default.
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• W1520592032 date "1973-07-01" @default.
• W1520592032 modified "2023-09-25" @default.
• W1520592032 title "Correlations Between Quantitative Assay of Red Cell-Bound C3, Serologic Reactions, and Hemolytic Anemia" @default.
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• W1520592032 doi "https://doi.org/10.4049/jimmunol.111.1.304.b" @default.
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