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- W1520632324 abstract "1. Partition of nucleic acids in liquid two-phase systems composed of two polymers, dextran and polyethylene glycol, and water is studied with respect to the addition of different electrolytes, polymer concentration and nucleic acid conformation. 2. The nucleic acids tested include native and heat denatured DNA from calf thymus, polyadenylic acid, polyuridylic acid, polycytidylic acid and polyinosinic acid. 3. Partition of nucleic acids depends highly on the electrolyte composition, both the ionic strength and the kinds of ions. A small shift in the ratio between two cations or two anions can thus transfer high-molecular-weight nucleic acids from one phase to the other. This transfer is specific for each type of nucleic acid. 4. The partition of single-stranded forms is compared with double or triple-stranded forms in systems with defined electrolyte compositions. It is demonstrated that a change from a single-stranded to double or triple-stranded forms is accompanied with an increase in the partition coefficient of 100–1000 times. 5. It is demonstrated that an 1:1 mixture of polyadenylic acid and polyinosinic acid results in the formation of a mixture of a three-stranded complex poly (adenylic acid + 2 inosinic acids) plus polyinosinic acid." @default.
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- W1520632324 date "1965-05-01" @default.
- W1520632324 modified "2023-10-14" @default.
- W1520632324 title "Partition studies on nucleic acids" @default.
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- W1520632324 doi "https://doi.org/10.1016/0005-2787(65)90537-x" @default.
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