Matches in SemOpenAlex for { <https://semopenalex.org/work/W1520656906> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1520656906 endingPage "4174" @default.
- W1520656906 startingPage "4169" @default.
- W1520656906 abstract "Subtilisin, an extracellular serine protease from Bacillus subtilis, requires the amino-terminal propeptide of 77 amino acid residues for the formation of the active enzyme. The propeptide is cleaved upon completion of folding. Serine 221 at the active center was substituted with cysteine, and the mutant enzyme (prothiolsubtilisin) was expressed in Escherichia coli under the control of a T7 promoter. Prothiosubtilisin, which was produced as inclusion bodies, was dissolved in 6 M guanidine HCl and purified to near homogeneity in the presence of 5 M urea. The purified protein was renatured by stepwise dialysis. In spite of the mutation at the active center, the propeptide was found to be autoprocessed with approximately 60-80% efficiency. However, protease activity could not be detected in the final product by the spectrophotometric assay. Moreover, the cleaved propeptide remained tightly bound to thiolsubtilisin without being digested, as evident by SDS-polyacrylamide gel electrophoresis. The amino-terminal sequence of the processed thiolsubtilisin was determined and proved that the propeptide was cleaved at a site identical to that of wild-type prosubtilisin. The processed thiolsubtilisin was also found to contain one free SH group/molecule. These results unambiguously demonstrate that the processing of prosubtilisin occurs by an intramolecular autoprocessing mechanism." @default.
- W1520656906 created "2016-06-24" @default.
- W1520656906 creator A5030465724 @default.
- W1520656906 creator A5040935882 @default.
- W1520656906 date "1994-02-01" @default.
- W1520656906 modified "2023-09-26" @default.
- W1520656906 title "Autoprocessing of prothiolsubtilisin E in which active-site serine 221 is altered to cysteine." @default.
- W1520656906 cites W1493907936 @default.
- W1520656906 cites W1541120458 @default.
- W1520656906 cites W1561041749 @default.
- W1520656906 cites W1563608009 @default.
- W1520656906 cites W1591438776 @default.
- W1520656906 cites W1612480077 @default.
- W1520656906 cites W1967278884 @default.
- W1520656906 cites W1967383104 @default.
- W1520656906 cites W1971022925 @default.
- W1520656906 cites W1980283608 @default.
- W1520656906 cites W1994646072 @default.
- W1520656906 cites W2005986251 @default.
- W1520656906 cites W2006370393 @default.
- W1520656906 cites W2032118018 @default.
- W1520656906 cites W2035246206 @default.
- W1520656906 cites W2045777307 @default.
- W1520656906 cites W2045786862 @default.
- W1520656906 cites W2045991415 @default.
- W1520656906 cites W2073647771 @default.
- W1520656906 cites W2090374619 @default.
- W1520656906 cites W2110455021 @default.
- W1520656906 cites W2134929446 @default.
- W1520656906 cites W2167546940 @default.
- W1520656906 cites W2167987924 @default.
- W1520656906 cites W90850719 @default.
- W1520656906 doi "https://doi.org/10.1016/s0021-9258(17)41758-3" @default.
- W1520656906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8307978" @default.
- W1520656906 hasPublicationYear "1994" @default.
- W1520656906 type Work @default.
- W1520656906 sameAs 1520656906 @default.
- W1520656906 citedByCount "53" @default.
- W1520656906 countsByYear W15206569062013 @default.
- W1520656906 countsByYear W15206569062016 @default.
- W1520656906 countsByYear W15206569062017 @default.
- W1520656906 countsByYear W15206569062019 @default.
- W1520656906 countsByYear W15206569062020 @default.
- W1520656906 countsByYear W15206569062022 @default.
- W1520656906 countsByYear W15206569062023 @default.
- W1520656906 crossrefType "journal-article" @default.
- W1520656906 hasAuthorship W1520656906A5030465724 @default.
- W1520656906 hasAuthorship W1520656906A5040935882 @default.
- W1520656906 hasBestOaLocation W15206569061 @default.
- W1520656906 hasConcept C11960822 @default.
- W1520656906 hasConcept C181199279 @default.
- W1520656906 hasConcept C185592680 @default.
- W1520656906 hasConcept C2776414213 @default.
- W1520656906 hasConcept C2779201268 @default.
- W1520656906 hasConcept C41183919 @default.
- W1520656906 hasConcept C55493867 @default.
- W1520656906 hasConcept C86803240 @default.
- W1520656906 hasConceptScore W1520656906C11960822 @default.
- W1520656906 hasConceptScore W1520656906C181199279 @default.
- W1520656906 hasConceptScore W1520656906C185592680 @default.
- W1520656906 hasConceptScore W1520656906C2776414213 @default.
- W1520656906 hasConceptScore W1520656906C2779201268 @default.
- W1520656906 hasConceptScore W1520656906C41183919 @default.
- W1520656906 hasConceptScore W1520656906C55493867 @default.
- W1520656906 hasConceptScore W1520656906C86803240 @default.
- W1520656906 hasIssue "6" @default.
- W1520656906 hasLocation W15206569061 @default.
- W1520656906 hasOpenAccess W1520656906 @default.
- W1520656906 hasPrimaryLocation W15206569061 @default.
- W1520656906 hasRelatedWork W2010913718 @default.
- W1520656906 hasRelatedWork W2016744971 @default.
- W1520656906 hasRelatedWork W2028357718 @default.
- W1520656906 hasRelatedWork W2042971445 @default.
- W1520656906 hasRelatedWork W2800095770 @default.
- W1520656906 hasRelatedWork W2952493855 @default.
- W1520656906 hasRelatedWork W4242569476 @default.
- W1520656906 hasRelatedWork W4249105471 @default.
- W1520656906 hasRelatedWork W2468457096 @default.
- W1520656906 hasRelatedWork W2741266057 @default.
- W1520656906 hasVolume "269" @default.
- W1520656906 isParatext "false" @default.
- W1520656906 isRetracted "false" @default.
- W1520656906 magId "1520656906" @default.
- W1520656906 workType "article" @default.