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- W1520686942 abstract "Abstract A mycobacterial glycolipid (P3) mixed with protein antigens in oil-in-water emulsion induced lasting delayed hypersensitivity (DH) and granulomatous inflammation after intradermal injection into guinea pigs. This did not occur when P3 and bovine serum albumin (BSA) were given in Freund's incomplete adjuvant. The oil-in-water emulsions consisted of microscopic oil droplets suspended in aqueous medium. By separating oil and aqueous phases from BSA + P3 emulsion it was shown that antigen retained with oil droplets led to DH and granuloma formation. The association of antigen with oil droplets was P3 dependent and was quantitated with 125I-labeled BSA. The same phenomenon occurred with 125I-labeled rabbit γ-globulin (RGG) + P3 emulsion. Fluorescein-conjugated RGG was observed in a particulate state within or on oil droplets in emulsion containing P3. These physical characteristics of antigen + P3 emulsion appeared to be important for immunogenicity." @default.
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- W1520686942 date "1976-02-01" @default.
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- W1520686942 title "Delayed Hypersensitivity and Granulomatous Response After Immunization with Protein Antigens Associated with a Mycobacterial Glycolipid and Oil Droplets" @default.
- W1520686942 doi "https://doi.org/10.4049/jimmunol.116.2.482" @default.
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