Matches in SemOpenAlex for { <https://semopenalex.org/work/W1520753273> ?p ?o ?g. }
- W1520753273 endingPage "1420" @default.
- W1520753273 startingPage "1414" @default.
- W1520753273 abstract "We and others have suggested previously that the binding of somatostatin to its receptors in the pancreas is regulated by not only somatostatin analogs but also cholecystokinin analogs in proportion to their known biological potencies. To clarify the precise mechanism by which unrelated peptides modulate somatostatin binding, the effect of a phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), or a synthetic diacylglycerol analog, 1-oleyl-2-acetylglycerol (OAG), on [125I-Tyr1]somatostatin binding to pancreatic acinar cell membranes was examined. Pretreatment of pancreatic acini for 120 min at 37 degrees C with 100 ng/ml TPA maximally reduced subsequent labeled somatostatin binding to acinar membranes. The inhibitory effect of TPA on the somatostatin binding was dependent on the dose used or the time and temperature of pretreatment. These effects of TPA were almost mimicked by the treatment of acini with OAG. Scatchard analysis of [125I-Tyr1]somatostatin binding demonstrated that the decrease in the labeled somatostatin binding induced by TPA or OAG pretreatment was due to the decrease in the maximum binding capacity without a significant change in the binding affinity. A specifically labeled single band of Mr = 90,000 obtained with a photoaffinity cross-linking study indicates that the somatostatin-binding sites are the same somatostatin receptor as previously described. Moreover, the intensity of the Mr = 90,000 band was dramatically decreased when acini were treated with increasing concentrations of TPA, a finding consistent with TPA-induced decrease in binding capacity. Such an inhibitory effect of TPA was abolished when pretreatment of acini with TPA was performed in the presence of Ca2+-chelating compounds such as EDTA and EGTA or phospholipid-interacting drugs such as chlorpromazine and tetracaine. Interestingly, the combined treatment of TPA and Ca2+ ionophore A23187 caused synergistic inhibition of the subsequent labeled somatostatin binding to acinar membranes, although Ca2+ ionophore itself almost failed to affect the somatostatin binding. These results suggest, therefore, that TPA or OAG can modulate somatostatin binding to its receptors on rat pancreatic acinar cell membranes, presumably through activation of Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C); and the activated protein kinase C and intracellular Ca2+ mobilization presumably act to modulate the pancreatic acinar somatostatin receptors synergistically." @default.
- W1520753273 created "2016-06-24" @default.
- W1520753273 creator A5022604285 @default.
- W1520753273 creator A5071680544 @default.
- W1520753273 creator A5073807260 @default.
- W1520753273 creator A5082560619 @default.
- W1520753273 date "1986-01-01" @default.
- W1520753273 modified "2023-09-30" @default.
- W1520753273 title "Phorbol ester or diacylglycerol modulates somatostatin binding to its receptors on rat pancreatic acinar cell membranes." @default.
- W1520753273 cites W131354686 @default.
- W1520753273 cites W1495646181 @default.
- W1520753273 cites W1495721887 @default.
- W1520753273 cites W1502848148 @default.
- W1520753273 cites W1524875319 @default.
- W1520753273 cites W1553799143 @default.
- W1520753273 cites W1594758412 @default.
- W1520753273 cites W1603813853 @default.
- W1520753273 cites W1604700543 @default.
- W1520753273 cites W1619707557 @default.
- W1520753273 cites W1672662520 @default.
- W1520753273 cites W1956721064 @default.
- W1520753273 cites W1963799051 @default.
- W1520753273 cites W1964747228 @default.
- W1520753273 cites W1983394026 @default.
- W1520753273 cites W1985690114 @default.
- W1520753273 cites W1998084952 @default.
- W1520753273 cites W2001692359 @default.
- W1520753273 cites W2011474783 @default.
- W1520753273 cites W2018515303 @default.
- W1520753273 cites W2019087981 @default.
- W1520753273 cites W2040380855 @default.
- W1520753273 cites W2047749839 @default.
- W1520753273 cites W2052643187 @default.
- W1520753273 cites W2055852252 @default.
- W1520753273 cites W2057032678 @default.
- W1520753273 cites W2077128471 @default.
- W1520753273 cites W2087574577 @default.
- W1520753273 cites W2088046252 @default.
- W1520753273 cites W2088199561 @default.
- W1520753273 cites W2092543377 @default.
- W1520753273 cites W2100837269 @default.
- W1520753273 cites W2119826412 @default.
- W1520753273 cites W2151335002 @default.
- W1520753273 cites W2161504149 @default.
- W1520753273 doi "https://doi.org/10.1016/s0021-9258(17)36108-2" @default.
- W1520753273 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2868000" @default.
- W1520753273 hasPublicationYear "1986" @default.
- W1520753273 type Work @default.
- W1520753273 sameAs 1520753273 @default.
- W1520753273 citedByCount "37" @default.
- W1520753273 crossrefType "journal-article" @default.
- W1520753273 hasAuthorship W1520753273A5022604285 @default.
- W1520753273 hasAuthorship W1520753273A5071680544 @default.
- W1520753273 hasAuthorship W1520753273A5073807260 @default.
- W1520753273 hasAuthorship W1520753273A5082560619 @default.
- W1520753273 hasBestOaLocation W15207532731 @default.
- W1520753273 hasConcept C126322002 @default.
- W1520753273 hasConcept C134018914 @default.
- W1520753273 hasConcept C150008808 @default.
- W1520753273 hasConcept C170493617 @default.
- W1520753273 hasConcept C185592680 @default.
- W1520753273 hasConcept C195794163 @default.
- W1520753273 hasConcept C2776297358 @default.
- W1520753273 hasConcept C2778764654 @default.
- W1520753273 hasConcept C2991930265 @default.
- W1520753273 hasConcept C3017695315 @default.
- W1520753273 hasConcept C32363146 @default.
- W1520753273 hasConcept C36020004 @default.
- W1520753273 hasConcept C41625074 @default.
- W1520753273 hasConcept C55493867 @default.
- W1520753273 hasConcept C62478195 @default.
- W1520753273 hasConcept C71924100 @default.
- W1520753273 hasConcept C80115893 @default.
- W1520753273 hasConcept C86803240 @default.
- W1520753273 hasConcept C95444343 @default.
- W1520753273 hasConceptScore W1520753273C126322002 @default.
- W1520753273 hasConceptScore W1520753273C134018914 @default.
- W1520753273 hasConceptScore W1520753273C150008808 @default.
- W1520753273 hasConceptScore W1520753273C170493617 @default.
- W1520753273 hasConceptScore W1520753273C185592680 @default.
- W1520753273 hasConceptScore W1520753273C195794163 @default.
- W1520753273 hasConceptScore W1520753273C2776297358 @default.
- W1520753273 hasConceptScore W1520753273C2778764654 @default.
- W1520753273 hasConceptScore W1520753273C2991930265 @default.
- W1520753273 hasConceptScore W1520753273C3017695315 @default.
- W1520753273 hasConceptScore W1520753273C32363146 @default.
- W1520753273 hasConceptScore W1520753273C36020004 @default.
- W1520753273 hasConceptScore W1520753273C41625074 @default.
- W1520753273 hasConceptScore W1520753273C55493867 @default.
- W1520753273 hasConceptScore W1520753273C62478195 @default.
- W1520753273 hasConceptScore W1520753273C71924100 @default.
- W1520753273 hasConceptScore W1520753273C80115893 @default.
- W1520753273 hasConceptScore W1520753273C86803240 @default.
- W1520753273 hasConceptScore W1520753273C95444343 @default.
- W1520753273 hasIssue "3" @default.
- W1520753273 hasLocation W15207532731 @default.
- W1520753273 hasOpenAccess W1520753273 @default.
- W1520753273 hasPrimaryLocation W15207532731 @default.