Matches in SemOpenAlex for { <https://semopenalex.org/work/W1520783034> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1520783034 endingPage "2529" @default.
- W1520783034 startingPage "2522" @default.
- W1520783034 abstract "To investigate the requirements for CD2 expression in the activation of T lymphocytes via the CD3-TCR complex, we produced and characterized a series of CD2-variants of the IL-2 producing Jurkat leukemia cell line, J32 (surface phenotype, CD2+, CD3+, CD28+). These mutants were derived by radiation and immunoselection, and were cloned under limiting dilution conditions. A total of 3 out of 30 of these mutants selectively lost the expression of both CD2 surface molecules and CD2 mRNA, and retained the expression of the CD3-TCR complex and the CD28 molecule. A mitogenic combination of anti-CD2 antibodies (9.6 + 9-1) failed to stimulate activation of these variants as measured by mobilization of intracellular Ca2+ and by IL-2 production. The CD2- mutants stimulated with anti-CD3 or anti-TCR mAb revealed an 8- to 32-fold decrease in IL-2 production and IL-2 mRNA accumulation as compared with the parental cells. No alteration of CD3-TCR-induced mobilization of intracellular Ca2+ was observed in the CD2- mutants. Reconstitution of CD2 expression by gene transfer in two J32 CD2- mutants restored IL-2 production and IL-2 mRNA accumulation in responses to both anti-CD2 and anti-CD3-TCR mAb. These results are the first direct demonstration of the requirement for CD2 molecules in optimizing IL-2 response in human T cells stimulated via CD3-TCR complex." @default.
- W1520783034 created "2016-06-24" @default.
- W1520783034 creator A5007782029 @default.
- W1520783034 creator A5013192956 @default.
- W1520783034 creator A5019678230 @default.
- W1520783034 creator A5041210699 @default.
- W1520783034 creator A5044774531 @default.
- W1520783034 creator A5049194943 @default.
- W1520783034 creator A5059438505 @default.
- W1520783034 creator A5082957375 @default.
- W1520783034 date "1991-04-15" @default.
- W1520783034 modified "2023-10-04" @default.
- W1520783034 title "Reconstitution of an active surface CD2 by DNA transfer in CD2-CD3+ Jurkat cells facilitates CD3-T cell receptor-mediated IL-2 production." @default.
- W1520783034 doi "https://doi.org/10.4049/jimmunol.146.8.2522" @default.
- W1520783034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1707910" @default.
- W1520783034 hasPublicationYear "1991" @default.
- W1520783034 type Work @default.
- W1520783034 sameAs 1520783034 @default.
- W1520783034 citedByCount "14" @default.
- W1520783034 countsByYear W15207830342013 @default.
- W1520783034 countsByYear W15207830342022 @default.
- W1520783034 crossrefType "journal-article" @default.
- W1520783034 hasAuthorship W1520783034A5007782029 @default.
- W1520783034 hasAuthorship W1520783034A5013192956 @default.
- W1520783034 hasAuthorship W1520783034A5019678230 @default.
- W1520783034 hasAuthorship W1520783034A5041210699 @default.
- W1520783034 hasAuthorship W1520783034A5044774531 @default.
- W1520783034 hasAuthorship W1520783034A5049194943 @default.
- W1520783034 hasAuthorship W1520783034A5059438505 @default.
- W1520783034 hasAuthorship W1520783034A5082957375 @default.
- W1520783034 hasConcept C114684123 @default.
- W1520783034 hasConcept C147483822 @default.
- W1520783034 hasConcept C148125776 @default.
- W1520783034 hasConcept C153911025 @default.
- W1520783034 hasConcept C167672396 @default.
- W1520783034 hasConcept C179464577 @default.
- W1520783034 hasConcept C19317047 @default.
- W1520783034 hasConcept C203014093 @default.
- W1520783034 hasConcept C2776090121 @default.
- W1520783034 hasConcept C86803240 @default.
- W1520783034 hasConcept C8891405 @default.
- W1520783034 hasConcept C95444343 @default.
- W1520783034 hasConceptScore W1520783034C114684123 @default.
- W1520783034 hasConceptScore W1520783034C147483822 @default.
- W1520783034 hasConceptScore W1520783034C148125776 @default.
- W1520783034 hasConceptScore W1520783034C153911025 @default.
- W1520783034 hasConceptScore W1520783034C167672396 @default.
- W1520783034 hasConceptScore W1520783034C179464577 @default.
- W1520783034 hasConceptScore W1520783034C19317047 @default.
- W1520783034 hasConceptScore W1520783034C203014093 @default.
- W1520783034 hasConceptScore W1520783034C2776090121 @default.
- W1520783034 hasConceptScore W1520783034C86803240 @default.
- W1520783034 hasConceptScore W1520783034C8891405 @default.
- W1520783034 hasConceptScore W1520783034C95444343 @default.
- W1520783034 hasIssue "8" @default.
- W1520783034 hasLocation W15207830341 @default.
- W1520783034 hasLocation W15207830342 @default.
- W1520783034 hasOpenAccess W1520783034 @default.
- W1520783034 hasPrimaryLocation W15207830341 @default.
- W1520783034 hasRelatedWork W102043283 @default.
- W1520783034 hasRelatedWork W1588650347 @default.
- W1520783034 hasRelatedWork W1602975132 @default.
- W1520783034 hasRelatedWork W1967977709 @default.
- W1520783034 hasRelatedWork W1995507496 @default.
- W1520783034 hasRelatedWork W2017019887 @default.
- W1520783034 hasRelatedWork W2024404265 @default.
- W1520783034 hasRelatedWork W2096118127 @default.
- W1520783034 hasRelatedWork W2153762242 @default.
- W1520783034 hasRelatedWork W4306155276 @default.
- W1520783034 hasVolume "146" @default.
- W1520783034 isParatext "false" @default.
- W1520783034 isRetracted "false" @default.
- W1520783034 magId "1520783034" @default.
- W1520783034 workType "article" @default.