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- W1520783480 abstract "D-Shikimic acids 1−14C, 6−14C and 7-(carboxyl) 14C have been biosynthetically prepared with specific activities of about 25 mCi/mmole and with overall yields of 45% based on pyruvate 3,2 or 1−14C as the respective starting materials. A crude cellfree homogenate of E. coli 83–24 was used to catalyze the condensation of phosphoenolpyruvate−14C with unlabelled erythrose-4-phosphate to give shikimic acid. Phosphoenolpyruvate−14C was synthesized from pyruvate−14C and ATP by the action of phosphoenolpyruvate-synthase. Erythrose-4-phosphate 1,2,3,4−14C and erythrose-4-phosphate-4-T was prepared from glucose-U−14C and glucose-6-T via enzymatic phosphorylation to labelled glucose-6-phosphate and subsequent lead tetraacetate oxidation. Condensation of labelled E-4-P with unlabelled PEP gave rise to shikimic acid-2,3,4,5−14C and 2-T." @default.
- W1520783480 created "2016-06-24" @default.
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- W1520783480 date "1971-10-01" @default.
- W1520783480 modified "2023-09-27" @default.
- W1520783480 title "Enzymic preparation of specifically14C and3H labelled shikimic acids" @default.
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- W1520783480 doi "https://doi.org/10.1002/jlcr.2590070419" @default.
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