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- W1520878034 abstract "Background The clinical usefulness of [−2]pro‐PSA (where PSA is prostate‐specific antigen) in prostate cancer diagnosis has been emphasized in recent studies. To determine proper blood sample handling conditions for [−2]pro‐PSA evaluation, we analyzed the preanalytical stability of [−2]pro‐PSA. Methods Blood samples from 22 Japanese males were stored under various conditions before total PSA (tPSA), free PSA, and [−2]pro‐PSA concentrations were measured, and the preanalytical stability of [−2]pro‐PSA and the changes in the Prostate Health Index ( phi ) were assessed. Results [−2]Pro‐PSA was stable in serum for at least 24 hr at both room temperature (RT) and at 4°C. However, [−2]pro‐PSA levels in whole blood increased rapidly over time, particularly at RT. Mean recovery (%) of [−2]pro‐PSA in whole blood at RT was >110% at 1 hr after drawing of blood. The phi tended to increase over time in a pattern similar to the change in[−2]pro‐PSA. Conclusions Preanalytical stability was lower for [−2]pro‐PSA than for free PSA or tPSA. Whole‐blood [−2]pro‐PSA increased in a time‐dependent manner, particularly at RT. Thus, whole blood samples collected at RT should be centrifuged within 1 hr after drawing. The [−2]pro‐PSA in serum is stable for at least 24 hr at both RT and at 4°C." @default.
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- W1520878034 date "2014-02-27" @default.
- W1520878034 modified "2023-10-04" @default.
- W1520878034 title "Stability of [−2]Pro-PSA in Whole Blood and Serum: Analysis for Optimal Measurement Conditions" @default.
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- W1520878034 doi "https://doi.org/10.1002/jcla.21687" @default.
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